AB59201

Anti-MiTF (phospho S73) antibody

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(7 제품이 사용된 논문 )

Rabbit Polyclonal MITF phospho S73 antibody. Suitable for WB and reacts with African green monkey samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MITF pS73 aa 150-200.

1 이미지

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Western blot - Anti-MiTF (phospho S73) antibody (AB59201)

All lanes:

Western blot - Anti-MiTF (phospho S73) antibody (ab59201) at 1/500 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract

Lane 2:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with phosphopeptide

Predicted band size: 58 kDa

Observed band size: 59 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

African green monkey

Applications

applications

Immunogen

Synthetic Peptide within Human MITF pS73 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

O75030

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR : in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed : 36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed : 36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758).

See full target information MITF pS73

대체 명칭 보기

BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of microbiology and biotechnology 34:985-986 PubMed38668686

2024

Corrigendum to: Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yujin Kim,Jung Ok Lee,Jung Min Lee,Mun-Hoe Lee,Hyeong-Min Kim,Hee-Chul Chung,Do-Un Kim,Jin-Hee Lee,Beom Joon Kim

Journal of microbiology and biotechnology 34:17-28 PubMed37830229

2023

Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yujin Kim,Jung Ok Lee,Jung Min Lee,Mun-Hoe Lee,Hyeong-Min Kim,Hee-Chul Chung,Do-Un Kim,Jin-Hee Lee,Beom Joon Kim

Journal of ginseng research 47:714-725 PubMed38107393

2023

Phenolic acids in inhibit melanin production through bidirectional regulation of melanin synthase transcription via different signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzeng Liu,Xiaohao Xu,Jingyuan Zhou,Guang Sun,Zhenzhuo Li,Lu Zhai,Jing Wang,Rui Ma,Daqing Zhao,Rui Jiang,Liwei Sun

The British journal of dermatology 185:391-404 PubMed33400324

2021

Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Y Lan,W Zeng,X Dong,H Lu

OncoTargets and therapy 13:12457-12469 PubMed33299329

2020

α-MSH-PE38KDEL Kills Melanoma Cells via Modulating Erk1/2/MITF/TYR Signaling in an MC1R-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Liu,Hong Li,Xianling Cong,Da Huo,Lele Cong,Guangzhi Wu

Oncotarget 8:95530-95541 PubMed29221146

2017

Swertiajaponin inhibits skin pigmentation by dual mechanisms to suppress tyrosinase.

Applications

Unspecified application

Species

Unspecified reactive species

Bonggi Lee,Kyoung Mi Moon,Bong-Seon Lee,Ju-Hye Yang,Kwang Il Park,Won-Kyung Cho,Jin Yeul Ma

Pathobiology : journal of immunopathology, molecul 82:269-79 PubMed26516773

2015

Evaluation of Fibroblast Growth Factor Receptor 2 Expression, Heterogeneity and Clinical Significance in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nayoung Han,Min A Kim,Hye Seung Lee,Woo Ho Kim

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MiTF (phospho S73) antibody

Western blot - Anti-MiTF (phospho S73) antibody (AB59201)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise