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AB247272

Anti-MK2 antibody [E341] - BSA and Azide free

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Rabbit Recombinant Monoclonal MK2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
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Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (AB247272)
  • WB

Unknown

Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (AB247272)

This data was developed using ab32567, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MK2 antibody [E341] (<a href='/ko/products/primary-antibodies/mk2-antibody-e341-ab32567'>ab32567</a>) at 1/1000 dilution

All lanes:

HeLa cell lysate

Predicted band size: 46 kDa

Observed band size: 46 kDa

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Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (AB247272)
  • WB

Lab

Western blot - Anti-MK2 antibody [E341] - BSA and Azide free (AB247272)

This data was developed using ab32567, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : MAPKAP Kinase 2knockout HAP1 cell lysate (20 μg)

Lane 3 : HeLa cell lysate (20 μg)

Lane 4 : A431 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MK2 antibody [E341] (<a href='/ko/products/primary-antibodies/mk2-antibody-e341-ab32567'>ab32567</a>)

Predicted band size: 46 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

E341

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises MAPKAP Kinase-2&#46;

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보완 제품

Sample Prep & Detection Kits

AB201797

Dylight® 350 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - Dylight® 350 Conjugation Kit (Fast) - Lightning-Link® (AB201797)

  • 0
  • 0
Sample Prep & Detection Kits

AB188290

Gold-Maleimide Conjugation Kit (40nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (40nm, 20 OD) (AB188290)

  • 0
  • 0
Primary Antibodies

AB119942

Anti-IRAK4 antibody [2H9]

KO Validated

Western blot - Anti-IRAK4 antibody [2H9] (AB119942)

  • 0
  • 0
Primary Antibodies

AB278705

Anti-MK2 (phospho T334) antibody [MAPKAPK2T334-H2]

20ul selling size|Recombinant

Flow Cytometry - Anti-MK2 (phospho T334) antibody [MAPKAPK2T334-H2] (AB278705)

  • 0
  • 0
Primary Antibodies

AB181553

Anti-IRF5 antibody [EPR17067]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-IRF5 antibody [EPR17067] (AB181553)

  • 3
  • 2
Primary Antibodies

AB32081

Anti-ERK2 antibody [E460]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ERK2 antibody [E460] (AB32081)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

ab247272 is the carrier-free version of ab32567.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MK2 protein also known as mitogen-activated protein kinase-activated protein kinase 2 or MAPKAPK2 is an important enzyme involved in cellular signaling. MK2 has a protein mass of approximately 44 kDa. It is widely expressed in various tissues indicating its broad functional roles. MK2 interacts with MAP kinases facilitating the transmission of signals within cells during stress responses and inflammation.
Biological function summary

MK2 plays an important role in the cellular stress response by regulating cytokine production particularly in inflammation. It acts as part of a protein complex with p38 MAPK enhancing its kinase activity. This interaction allows MK2 to control the expression of inflammatory cytokines and other stress-responsive proteins. The complex also responds to cellular stress signals allowing it to modulate gene expression effectively.

Pathways

MK2 contributes to the p38 MAPK signaling pathway a major route for stress and inflammatory responses in cells. MK2 often interacts with related proteins like p38 MAPK which are directly upstream in the signaling cascade. Additionally MK2 is involved in the post-transcriptional regulation of mRNA stability and translation interfacing with the NF-kB signaling pathway highlighting its regulatory role in inflammation.

MK2's role in inflammation links it to numerous inflammatory diseases such as rheumatoid arthritis and Crohn's disease. MK2's interaction with other proteins like p38 MAPK and downstream effectors such as transcription factors emphasizes its therapeutic potential. Targeting MK2 and its interaction with these proteins may offer novel treatments for conditions characterized by dysregulated inflammatory responses.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed : 16278218). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally : acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed : 26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization : following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells : required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
See full target information MAPKAPK2

대체 명칭 보기

MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2, MAPKAPK2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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