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AB243737

Anti-MLC1SA antibody

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Rabbit Polyclonal MLC1SA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MYL6B aa 1-100.

대체 명칭 보기

MLC1SA, MYL6B, Myosin light chain 6B, Myosin light chain 1 slow-twitch muscle A isoform, Smooth muscle and nonmuscle myosin light chain alkali 6B, MLC1sa

4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLC1SA antibody (AB243737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLC1SA antibody (AB243737)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for MLC1SA with ab243737 at a 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLC1SA antibody (AB243737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLC1SA antibody (AB243737)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for MLC1SA with ab243737 at a 1/20 dilution in immunohistochemical analysis. Low expression as expected.

Immunocytochemistry/ Immunofluorescence - Anti-MLC1SA antibody (AB243737)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MLC1SA antibody (AB243737)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling MLC1SA using ab243737 at 4 μg/ml (green) in ICC/IF.

Western blot - Anti-MLC1SA antibody (AB243737)
  • WB

Supplier Data

Western blot - Anti-MLC1SA antibody (AB243737)

All lanes:

Western blot - Anti-MLC1SA antibody (ab243737) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

MLC1SA overexpression HEK-293T lysate

Predicted band size: 23 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MYL6B aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P14649

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보완 제품

Primary Antibodies

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Anti-Caspase-3 antibody [EPR18297]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

  • 3
  • 3
Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

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Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
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Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MLC1SA also known as myosin light chain 1 slow-twitch muscle isoform plays a significant role in muscle contraction. It binds to myosin heavy chains in muscle fibers regulating the actin-myosin interaction essential for muscle contraction. MLC1SA has a molecular mass of approximately 18 kDa and shows expression predominantly in slow-twitch muscle fibers which are important for endurance activities. It also exhibits expression in certain cardiac tissues highlighting its function beyond skeletal muscles.
Biological function summary

MLC1SA contributes to muscle contraction regulation by modulating the interaction between actin and myosin. It is part of a complex that includes myosin heavy chains and other associated proteins. Through phosphorylation events MLC1SA adjusts the sensitivity of muscle fibers to calcium which is critical for the fine-tuning of muscle responses to stimuli. This subtle modulation is important for activities that rely on sustained muscle tension.

Pathways

Myosin light chain signaling involves MLC1SA as a central component in muscular contraction pathways. It plays a role in the myosin-actin crossbridge cycle aiding the efficient conversion of ATP into mechanical work. MLC1SA interacts with other proteins such as myosin heavy chains and calmodulin. These interactions are critical in pathways like the calcium signaling pathway and muscle contraction pathway where calcium ions serve as triggers for changes in muscle cell activity.

Defects or alterations in MLC1SA expression or function can relate to muscular dystrophies and myopathies. These conditions often involve muscle weakness and reduced endurance where MLC1SA disruption alters normal muscle function. Moreover cardiomyopathies can show links with this protein especially when considering the interaction with myosin heavy chains and tropomyosin. Alterations in these interactions may disrupt cardiac muscle contraction contributing to disease pathogenesis.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Regulatory light chain of myosin. Does not bind calcium.
See full target information MYL6B
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

American journal of reproductive immunology (New York, N.Y. : 1989) 94:e70132 PubMed40815040

2025

ATP/P2X4 Regulates Inflammation and Oxidative Stress in Endometriosis Through NLRP3 Inflammasome-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wu,Yan Guo
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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