AB243142

Anti-MLKL antibody [3H1]

Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(47 제품이 사용된 논문 )

Rat Recombinant Monoclonal MLKL antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 47 publications.

대체 명칭 보기

Mixed lineage kinase domain-like protein, hMLKL, MLKL

1 이미지

Supplier Data

Western blot - Anti-MLKL antibody [3H1] (AB243142)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-3 : 5 secs; Lanes 4/5/7 : 3 secs; Lane 6 : 15 secs.

All lanes:

Western blot - Anti-MLKL antibody [3H1] (ab243142) at 1/2000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell), whole cell lysate at 10 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Lane 5:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 7:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/2000 dilution

Predicted band size: 54 kDa

false

주요 정보

Host species

Rat

Clonality

Monoclonal

Clone number

3H1

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" } } }

style

left-column

제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MLKL also known as mixed lineage kinase domain-like protein plays a critical role in the process of necroptosis a form of programmed cell death. The MLKL protein has a molecular weight of approximately 54 kDa. The protein exists mainly within the cytoplasm but translocates to the plasma membrane during cell death execution. Expression of MLKL happens in various tissues indicating its wide biological importance. Phosphorylation of MLKL often referred to as p-MLKL is key to triggering its activity marking the transition from an inactive to an active state during necroptosis.

Biological function summary

The MLKL protein acts as an executioner of cell death by forming a complex that disrupts the plasma membrane integrity. This process is downstream of receptor-interacting serine/threonine-protein kinase 3 (RIPK3) which phosphorylates MLKL to form the active necrosome complex. Active MLKL oligomerizes and migrates towards the inner leaflet of the plasma membrane binding to phosphatidylinositol phosphates which assists in pore formation and cellular rupture. The ability to measure MLKL activity levels such as via MLKL ELISA kits is important for understanding necrotic processes in detailed studies.

Pathways

MLKL is integrally involved in the necroptotic pathway alongside RIPK1 and RIPK3 which are key initiators of necroptosis. Phosphorylated MLKL acts downstream of RIPK3 resulting in cell death without caspase activation distinguishing necroptosis from apoptosis. MLKL and RIPK3 are tightly linked within this pathway with MLKL phosphorylation serving as a vital event for the execution phase. The necroptosis pathway is part of larger networks including inflammatory response pathways highlighting the importance of MLKL's role beyond sheer cell death.

MLKL has been implicated in various inflammatory conditions and neurodegenerative diseases. The dysregulation of necroptosis can contribute to disorders such as inflammatory bowel disease and amyotrophic lateral sclerosis. In inflammatory bowel disease increased levels of p-MLKL might lead to excessive cell death exacerbating inflammation. Similarly in neurodegenerative disorders the harmful activation of MLKL may accelerate neuronal cell death. Key interactions with proteins like RIPK3 and RIPK1 highlight MLKL's involvement in these pathological processes making it a potential target for therapeutic intervention.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed : 22265413, PubMed : 22265414, PubMed : 22421439, PubMed : 24316671). Does not have protein kinase activity (PubMed : 22265413, PubMed : 22265414, PubMed : 22421439, PubMed : 24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed : 22265413, PubMed : 22265414, PubMed : 22421439, PubMed : 24316671). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection : following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (By similarity). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed : 29883610).

See full target information MLKL

style

left-column

제품이 사용된 논문 (47)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomolecules 15: PubMed40563424

2025

Caffeic Acid Phenethyl Ester Protects Against Doxorubicin-Induced Cardiotoxicity via Inhibiting the ROS-MLKL-Mediated Cross-Talk Between Oxidative Stress and Necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenying Jiang,Tinghuang Zhang,Jiawen Gu,Chenjun Shen,Hang Gao,Hai An,Chen Wang,Jiahui Lu,Shengzhang Lin,Huajun Zhao,Zhihui Zhu

PLoS pathogens 21:e1013167 PubMed40359428

2025

Caspase-1-licensed pyroptosis drives dsRNA-mediated necroptosis and dampens host defense against bacterial pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Qinyu Luo,Lihua Shen,Shiyue Yang,Yan Zhang,Yihang Pan,Zehua Wu,Qiang Shu,Qixing Chen

The EMBO journal 44:2424-2445 PubMed40128366

2025

A bacterial network of T3SS effectors counteracts host pro-inflammatory responses and cell death to promote infection.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wen Yeap,Ghin Ray Goh,Safwah Nasuha Rosli,Hai Shin Pung,Cristina Giogha,Vik Ven Eng,Jaclyn S Pearson,Elizabeth L Hartland,Kaiwen W Chen

British journal of pharmacology 182:2772-2789 PubMed40064624

2025

SEW2871 attenuates myocyte necroptosis in heart failure through inhibition of oxidative stress and inflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Xia Guo,Run-Nan Tantai,Bin Yang,Li-Guo Yang,Yuan Ma,Hui-Ping Zhao,Jing Wang,Xiao-Juan Zhang,Rui-Hua Wang,Fei Wang,Jia-Pu Wang,Rui-Fang Chi,Fu-Zhong Qin,Bao Li,Ya-Xin Liu

Frontiers in medicine 12:1521729 PubMed40061378

2025

Inhibition of renin-angiotensin system attenuates type I alveolar epithelial cell necroptosis in rats after hyperbaric hyperoxic exposure.

Applications

Unspecified application

Species

Unspecified reactive species

CuiHong Han,PeiXi Zhang,Ying Liu,JiaJun Xu,XuHua Yu,YuKun Wen,ShiFeng Wang,WenWu Liu

The Tohoku journal of experimental medicine : PubMed39938906

2025

Fructus Gardenia Extract Improves Cerebral Ischemia-Reperfusion Injury via RIPK1-RIPK3-MLKL Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shengyu Jin,Ke Wang,Zhangfeng Shen,Bing Hu,Qian Dou,Dongdong Xu,Yuhao Chen,Jiamin Yan,Jing Liu

The FEBS journal 292:1972-1990 PubMed39827378

2025

TNFAIP3-interacting protein 1 (ABIN-1) negatively regulates caspase-8/FADD-dependent pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyi Li,Daoyong Wang,Zhenyi Su,Xiaohua Mao

Mediators of inflammation 2024:1995952 PubMed39669913

2024

Peptide BG From Bitter Gourd () Improves Adjuvant-Induced Arthritis by Modulating the Necroptosis/Neutrophil Extracellular Traps/Inflammation Axis and the Gut Microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyan Han,Yanan Xu,Suyila Qimuge,Changshan Wang,Xiulan Su

Acta biochimica et biophysica Sinica 56:1537-1548 PubMed39314165

2024

Unveiling the cytotoxicity of a new gold(I) complex towards hepatocellular carcinoma by inhibiting TrxR activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Wang,Haokun Yuan,Ruiqin Fang,Ran Zhang,Wei-Jia Wang

Renal failure 46:2403649 PubMed39311631

2024

protein targeted activation of -mediated PANoptosis promotes sepsis-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Siwei Wei,Lei Wu,Zhen Xiang,Xiaoxiao Yang,Dongjie Pei,Liubing Jiang,Zhen Du

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MLKL antibody [3H1]

Western blot - Anti-MLKL antibody [3H1] (AB243142)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (47)

Product promise