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AB326659

Anti-MLKL (phospho S358) antibody [SR1963]

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Rabbit Recombinant Monoclonal MLKL phospho S358 antibody. Suitable for WB, IHC-P and reacts with Human samples.
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Western blot - Anti-MLKL (phospho S358) antibody [SR1963] (AB326659)
  • WB

Supplier Data

Western blot - Anti-MLKL (phospho S358) antibody [SR1963] (AB326659)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : HT-29 cell lysate
Lane 2 : HT-29 cell lysate treated with 20 ng/ml TNF-a, 100 nM SM-164 and 20 µM z-VAD
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 54 kDa
Observed band size : 54 kDa
Exposure time : 9 seconds

All lanes:

Western blot - Anti-MLKL (phospho S358) antibody [SR1963] (ab326659) at 1/1000 dilution

Lane 1:

HT-29 cell lysate at 20 µg

Lane 2:

HT-29 cell lysate treated with 20 ng/ml TNF-a, 100 nM SM-164 and 20 µM z-VAD at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Exposure time: 9s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1963

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB196436

Anti-MLKL (phospho S345) antibody [EPR9515(2)]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-MLKL (phospho S345) antibody [EPR9515(2)] (AB196436)

  • 4
  • 27
Primary Antibodies

AB209384

Anti-RIP3 (phospho S227) antibody [EPR9627]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-RIP3 (phospho S227) antibody [EPR9627] (AB209384)

  • 0
  • 0
Primary Antibodies

AB316957

Anti-RIP3 antibody [EPR24374-9]

20ul selling size|Recombinant|RabMAb

Immunoprecipitation - Anti-RIP3 antibody [EPR24374-9] (AB316957)

  • 0
  • 0
Primary Antibodies

AB316923

Anti-RIP (phospho S166) antibody [EPR25654-166]

20ul selling size|Recombinant|RabMAb|KO Validated

Western blot - Anti-RIP (phospho S166) antibody [EPR25654-166] (AB316923)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed : 22265413, PubMed : 22265414, PubMed : 22421439, PubMed : 24316671). Does not have protein kinase activity (PubMed : 22265413, PubMed : 22265414, PubMed : 22421439, PubMed : 24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed : 22265413, PubMed : 22265414, PubMed : 22421439, PubMed : 24316671). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection : following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (By similarity). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed : 29883610).
See full target information MLKL pS358

대체 명칭 보기

Mixed lineage kinase domain-like protein, hMLKL, MLKL
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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