JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB192582

Anti-MLL5 antibody

4

(1 리뷰)

|

(1 출판물)

Rabbit Polyclonal MLL5 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KMT2E.

대체 명칭 보기

MLL5, KMT2E, Inactive histone-lysine N-methyltransferase 2E, Inactive lysine N-methyltransferase 2E, Myeloid/lymphoid or mixed-lineage leukemia protein 5

1 이미지
Western blot - Anti-MLL5 antibody (AB192582)
  • WB

Supplier Data

Western blot - Anti-MLL5 antibody (AB192582)

All lanes:

Western blot - Anti-MLL5 antibody (ab192582) at 1/500 dilution

Lane 1:

NCI-H460 (human lung cancer cell line) whole cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 3:

Mouse brain tissue lysate

Predicted band size: 205 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KMT2E.

Q8IZD2

style
left-column

보완 제품

Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

  • 5
  • 90
Assay Kits

AB133053

Serotonin ELISA Kit

Competitive ELISA - Serotonin ELISA Kit (AB133053)

  • 4
  • 5
Primary Antibodies

AB209402

Anti-SF3B3 antibody [EPR18440]

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-SF3B3 antibody [EPR18440] (AB209402)

  • 0
  • 0
Primary Antibodies

AB223521

Anti-NPL antibody

Immunocytochemistry/ Immunofluorescence - Anti-NPL antibody (AB223521)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab192582 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
보관 버퍼
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MLL5 also known as KMT2E is a member of the mixed-lineage leukemia (MLL) family of histone methyltransferases and it is a protein with a mass of approximately 210 kDa. The protein is expressed in various human tissues with higher levels noted in the brain and testis. MLL5 functions mechanically by adding methyl groups to histone H3 at lysine 4 (H3K4) which plays a significant role in chromatin remodeling and regulating gene expression.
Biological function summary

MLL5 participates in the modulation of gene transcription influencing cell cycle progression and differentiation. This protein associates with other proteins to form multi-protein complexes that regulate epigenetic modifications and influence gene expression patterns necessary for development and proliferation. MLL5 also interacts with various transcription factors contributing to cellular responses during immune system development and function.

Pathways

The protein MLL5 plays a critical role in various signaling pathways including the Wnt signaling and the DNA repair pathways. It associates with proteins like β-catenin in the Wnt pathway and works with the protein P53 in DNA damage response and repair. These interactions help maintain genomic stability and proper cellular responses to developmental cues and stress.

Alterations in MLL5 expression impact conditions like acute myeloid leukemia (AML) and neurodegenerative diseases. In AML MLL5 interactions with other regulatory proteins like AML1/ETO influence cell growth and differentiation pathways contributing to disease pathology. In neurodegenerative conditions MLL5 dysregulation affects neuronal survival and function potentially through pathways involving tau proteins. Understanding these connections helps develop therapeutic strategies for these disorders.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription (PubMed : 23629655, PubMed : 23798402, PubMed : 24130829). Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3) (PubMed : 23798402, PubMed : 24130829). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation (By similarity). Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry (PubMed : 14718661, PubMed : 18573682, PubMed : 19264965, PubMed : 23629655). Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition (PubMed : 23629655). During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells (By similarity).. Isoform NKp44L. Cellular ligand for NCR2/NKp44, may play a role as a danger signal in cytotoxicity and NK-cell-mediated innate immunity.
See full target information KMT2E
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine reports 18:308-314 PubMed29749526

2018

Human HLA‑F adjacent transcript 10 promotes the formation of cancer initiating cells and cisplatin resistance in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Li,Zhenfan Wang,Ninghan Feng,Jian Dong,Xiaoyan Deng,Yin Yue,Yuehong Guo,Jianquan Hou
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com