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AB65147

Anti-MLX antibody

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Rabbit Polyclonal MLX antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MLX.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-MLX antibody (AB65147)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MLX antibody (AB65147)

ICC/IF image of ab65147 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65147, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-MLX antibody (AB65147)
  • WB

Unknown

Western blot - Anti-MLX antibody (AB65147)

All lanes:

Western blot - Anti-MLX antibody (ab65147) at 1/500 dilution

Lane 1:

LOVO cell extract at 5 µg

Lane 2:

LOVO cell extract at 5 µg with immunising peptide

Predicted band size: 33 kDa

Observed band size: 33 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human MLX. The exact immunogen used to generate this antibody is proprietary information.

Q9UH92

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보완 제품

Primary Antibodies

AB199489

Anti-MAX antibody [EPR19352] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

ChIP-sequencing - Anti-MAX antibody [EPR19352] - ChIP Grade (AB199489)

  • 5
  • 3
Primary Antibodies

AB313741

Anti-Lrp2 / Megalin antibody [EPR26093-68]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Lrp2 / Megalin antibody [EPR26093-68] (AB313741)

  • 0
  • 0
Primary Antibodies

AB224491

Anti-Synaptopodin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Synaptopodin antibody (AB224491)

  • 5
  • 3
Primary Antibodies

AB272544

Anti-TRIB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TRIB2 antibody (AB272544)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MLX also known as Max-like protein is a basic helix-loop-helix leucine zipper transcription factor. It has a molecular mass of approximately 31 kDa. MLX expresses in various tissues with prominent expression in metabolic organs such as the liver. This protein is part of the larger Mondo family and interacts with other Max network transcription bHLH proteins. MLX functions as a binding partner to form heterodimers which then bind to DNA at E-box sequences to regulate gene transcription.
Biological function summary

MLX regulates cellular energy homeostasis and glucose metabolism. It partners with Mondo proteins such as MondoA to form transcriptional complexes that control the expression of glycolytic enzymes and other metabolic genes. This role is essential for cellular adaptation to changes in nutrient availability and energy demands. MLX's function influences the cell's metabolic adaptation and its overall contribution to energy balance in the body.

Pathways

MLX engages with the glucose signaling pathway and the pentose phosphate pathway. It interacts with enzymes and proteins like glucose-6-phosphate dehydrogenase through transcriptional regulation. By forming dimeric complexes MLX controls the transcriptional responses to cellular glucose levels coordinating with other transcription factors such as c-Myc. This coordination helps in maintaining metabolic homeostasis and fosters cellular growth and proliferation under varying nutrient states.

Scientists link MLX to metabolic disorders and cancer. Dysregulation of MLX-related pathways may lead to metabolic diseases such as diabetes where impaired glucose signaling is a concern. Moreover MLX's interaction with proteins like c-Myc positions it as a modifier in cancer progression as aberrant transcriptional control can drive oncogenic processes. Understanding MLX's role in these contexts aids in identifying therapeutic targets for associated diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
See full target information MLX

대체 명칭 보기

BHLHD13, TCFL4, MLX, Max-like protein X, Class D basic helix-loop-helix protein 13, Max-like bHLHZip protein, Protein BigMax, Transcription factor-like protein 4, bHLHd13
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Neuron 92:1294-1307 PubMed28009275

2016

The Mammalian-Specific Protein Armcx1 Regulates Mitochondrial Transport during Axon Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Romain Cartoni,Michael W Norsworthy,Fengfeng Bei,Chen Wang,Siwei Li,Yiling Zhang,Christopher V Gabel,Thomas L Schwarz,Zhigang He
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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