Anti-MMP1 antibody [EP1249Y] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (AB240086)

This data was developed using ab134184, the same antibody clone in a different buffer formulation. Immunocytochemistry/ Immunofluorescence analyis of HUVEC (human umbilical vein endothelial cell) labeling MMP1 with at ab134184 at 1/100 followed by ab150081 at 1/1000 dilution as the secondary antibody. ab195889 at 1/200 was used as counterstain. Cells were fixed with 4% Paraformaldehyde and permeabilised with  0.1% TritonX-100. Nuclear counterstain was DAPI (blue). Confocal image showing cytoplasmic staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Low expression : A549. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IP

Lab

Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (AB240086)

This data was developed using ab134184, the same antibody clone in a different buffer formulation.

MMP1 was immunoprecipitated from 0.35 mg MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg with 134184 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab134184 IP in MDA-MB-231 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab134184 in MDA-MB-231 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MMP1 antibody [EP1249Y] (<a href='/ko/products/primary-antibodies/mmp1-antibody-ep1249y-ab134184'>ab134184</a>)

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

• WB

Unknown

Western blot - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (AB240086)

This data was developed using ab134184, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MMP1 antibody [EP1249Y] (<a href='/ko/products/primary-antibodies/mmp1-antibody-ep1249y-ab134184'>ab134184</a>) at 1/1000 dilution

All lanes:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 54 kDa

false

• WB

Unknown

Western blot - Anti-MMP1 antibody [EP1249Y] - BSA and Azide free (AB240086)

This data was developed using ab134184, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MMP1 antibody [EP1249Y] (<a href='/ko/products/primary-antibodies/mmp1-antibody-ep1249y-ab134184'>ab134184</a>) at 1/500 dilution

Lane 1:

MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate at 10 µg

Lane 2:

Lysate from HeLa cells untreated with TPA at 10 µg

Lane 3:

HeLa treated with 100 ng/mL TPA for 10 hours cell lysate at 10 µg

Lane 4:

Human colon cancer tissue lysate at 10 µg

Lane 5:

Human breast cancer tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Exposure time: 30s