Anti-MMP16 antibody [RP23040073]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MMP16 Antibody [RP23040073] (AB313410)

Immunofluorescent analysis of 70% confluent log phase PC-3 cells fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature labeling MMP16 with ab313410 at 1 µg/mL concentration followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 at 1/2000 dilution, for 45 minutes at room temperature (Panel a : Green). Nuclei (Panel b : Blue) were stained with DAPI. F-actin (Panel c : Red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MMP16 Antibody [RP23040073] (AB313410)

Immunofluorescent analysis of HeLa cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permeabilized with 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling MMP16 with ab313410 followed by detection using an Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor® 594 phalloidin (red) (image C). Image D is a composite image showing membrane localization of MMP-16.

• Flow Cyt

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Flow Cytometry - Anti-MMP16 Antibody [RP23040073] (AB313410)

Flow cytometric analysis of HeLa cells fixed and permeabilized labeling MMP16 with ab313410 (right) compared with a Isotype control (left). Secondary antibody : Alexa Fluor® 488 Goat anti-Rabbit IgG.

• IHC-P

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Immunohistochemistry paraffin embedded sections - Anti-MMP16 Antibody [RP23040073] (AB313410)

Immunohistochemical analysis of paraffin-embedded human placenta tissue (right) compared to a negative control without primary antibody (left) labeling MMP16 with ab313410 at 1/100 dilution followed by a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Counter stained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS.

• WB

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Western blot - Anti-MMP16 Antibody [RP23040073] (AB313410)

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Western blot - Anti-MMP16 Antibody [RP23040073] (ab313410) at 1 µg/mL

All lanes:

Jurkat whole cell extract

Observed band size: 52 kDa

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