AB270246

Anti-MMP9 antibody [2C3]

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Mouse Monoclonal MMP9 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MMP9 aa 600-650.

대체 명칭 보기

CLG4B, MMP9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [2C3] (AB270246)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [2C3] (AB270246)

Formalin-fixed, paraffin-embedded human spleen stained for MMP9 using ab270246 at 2μg/ml in imunnohistochemical analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2C3

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MMP9 aa 600-650. The exact immunogen used to generate this antibody is proprietary information.

P14780

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대체품

Primary Antibodies

AB137867

Anti-MMP9 antibody [EP1255Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] (AB137867)

Primary Antibodies

AB219372

Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [EP1255Y] - Low endotoxin, Azide free (AB219372)

Primary Antibodies

AB237782

Anti-MMP9 antibody [EPR22140-154] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EPR22140-154] - BSA and Azide free (AB237782)

Primary Antibodies

AB76003

Anti-MMP9 antibody [EP1254]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-MMP9 antibody [EP1254] (AB76003)

Primary Antibodies

AB228402

Anti-MMP9 antibody [EPR22140-154]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-MMP9 antibody [EPR22140-154] (AB228402)

Primary Antibodies

AB204850

Anti-MMP9 antibody [EP1254] - BSA and Azide free

RabMAb|Recombinant

Functional Studies - Anti-MMP9 antibody [EP1254] - BSA and Azide free (AB204850)

보완 제품

Assay Kits

AB246539

Human MMP9 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MMP9 ELISA Kit (AB246539)

Primary Antibodies

AB86607

Anti-MMP2 antibody [6E3F8]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP2 antibody [6E3F8] (AB86607)

Primary Antibodies

AB9324

Anti-IL-6 antibody [1.2-2B11-2G10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (AB9324)

Primary Antibodies

AB32124

Anti-Bcl-2 antibody [E17]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [E17] (AB32124)

Primary Antibodies

AB6994

Anti-Von Willebrand Factor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (AB6994)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).

Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed : 12879005, PubMed : 1480034, PubMed : 2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed : 12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed : 32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed : 1480034). Degrades fibronectin but not laminin or Pz-peptide.

See full target information MMP9

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Hepatology communications 9: PubMed40366121

2025

Single-cell transcription reveals hepatocyte-to-cholangiocyte reprogramming and biliary gene profile in biliary atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdu Meng,Min Du,Haodong Li,Fanyang Kong,Jiajian Yang,Rui Dong,Shan Zheng,Gong Chen,Zhen Shen,Junfeng Wang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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