AB154802

Anti-MNAT1 antibody [EPR2555(2)]

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal MNAT1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

대체 명칭 보기

CAP35, MAT1, RNF66, MNAT1, CDK-activating kinase assembly factor MAT1, CDK7/cyclin-H assembly factor, Cyclin-G1-interacting protein, Menage a trois, RING finger protein 66, RING finger protein MAT1, p35, p36

3 이미지

Flow Cytometry (Intracellular) - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Intracellular flow cytometric analysis of permeabilized Hela cells labeling MAT1 with ab154802 at 1/10 dilution (red) or a negative rabbit IgG (green).

Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Immunofluorescence analysis of HeLa cells labeling MAT1 with ab154802 at 1/100 dilution.

Unknown

Western blot - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

All lanes:

Western blot - Anti-MNAT1 antibody [EPR2555(2)] (ab154802) at 1/1000 dilution

Lane 1:

A431 lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

A673 lysate at 10 µg

Lane 4:

RAW264.7 lysate at 10 µg

Predicted band size: 36 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2555(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein MNAT1 also known as menage à trois 1 functions as a component of the CDK-activating kinase complex. It weighs about 32 kilodaltons. MNAT1 plays a role in regulating cell cycle progression. It expresses widely in various tissues but shows higher levels in proliferating tissues due to its involvement in cell division. Its expression pattern suggests a significant function in maintaining proper cell cycle control.

Biological function summary

This protein serves as a regulatory subunit of the CDK7-cyclin H-MNAT1 complex. This complex forms the core of CDK-activating kinase which is essential for the activation of other cyclin-dependent kinases (CDKs). By doing so MNAT1 influences transcription regulation and DNA repair processes. As part of this complex MNAT1 modulates the activity of the proteins involved in a variety of cellular mechanisms. Through these activities MNAT1 helps in maintaining genomic integrity and proper cellular response to DNA damage.

Pathways

The activity of MNAT1 is involved in the cell cycle regulation pathway and the transcription regulation process. It plays a role in phosphorylating other CDKs directly influencing their activation and consequently cell cycle control. MNAT1 associates closely with proteins such as cyclin H and CDK7 within these pathways. This collaboration ensures that the essential checkpoints in the cell cycle are correctly regulated and that transcription processes are executed properly.

Irregularities in MNAT1 function can correlate with cancer development due to its central role in cell cycle regulation and genomic stability. Altered expression levels or mutations in MNAT1 can disrupt normal cell division leading to unchecked cell proliferation. Furthermore MNAT1 interacts with cyclin H and CDK7 which are also important in the proper regulation of cell cycle checkpoints and can also influence cancer pathways when dysfunctional. Hence MNAT1 is a potential target for therapeutic strategies aiming to control abnormal cell growth.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

See full target information MNAT1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular biology of the cell 29:1111-1124 PubMed29496966

2018

C3G dynamically associates with nuclear speckles and regulates mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Dhruv Kumar Shakyawar,Bhattiprolu Muralikrishna,Vegesna Radha

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MNAT1 antibody [EPR2555(2)]

Flow Cytometry (Intracellular) - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

Western blot - Anti-MNAT1 antibody [EPR2555(2)] (AB154802)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise