AB324847

Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Crimean-Congo hemorrhagic fever virus (isolate C68031), Recombinant fragment - Crimean-Congo hemorrhagic fever virus (isolate C68031) samples.

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Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

This data was developed using ab323368, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab323368 at 2000 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Lysate of 293T cells transfected with a Crimean-Congo hemorrhagic fever virus Nucleoprotein, Dugbe virus (isolate ArD44313) Nucleoprotein, Hazara virus (isolate JC280) Nucleoprotein expression vector containing a myc-His-tag.

Antigen concentration : 1000-0 ug/ml

I-ELISA

Supplier Data

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

This data was developed using ab323368, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab323368 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : recombinant Crimean-Congo hemorrhagic fever virus Nucleoprotein.

Antigen concentration : 5000 ng/ml

Supplier Data

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

This data was developed using ab323368, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] (<a href='/ko/products/primary-antibodies/mouse-crimean-congo-hemorrhagic-fever-virus-nucleoprotein-antibody-a4-ab323368'>ab323368</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a Crimean-Coo hemorrhagic fever virus Nucleoprotein expression vector containi a myc-His-tag® whole cell lysate at 4 µg

Lane 3:

293T cells transfected with a Dbe virus (isolate ArD44313) Nucleoprotein expression vector containing a myc-His-tag® whole cell lysate at 4 µg

Lane 4:

293T cells transfected with a Hazara virus (isolate JC280) Nucleoprotein expression vector containi a myc-His-tag® whole cell lysate at 4 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 54 kDa,36 kDa,37-50 kDa

false

Exposure time: 3s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Carrier free

Yes

Reacts with

Crimean-Congo hemorrhagic fever virus (isolate C68031)

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell lysate - Crimean-Congo hemorrhagic fever virus (isolate C68031)": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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제품 세부 정보

ab324847 is the carrier-free version of ab323368.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds dsRNA and ssRNA and probably participates in the packaging of viral genome (By similarity). In the dsRNA binding mode, the nucleocapsid protein specifically binds to the vRNA panhandle secondary structure formed at the termini of viral genome (By similarity). Does not discriminate between viral and nonviral RNAs through ssRNA binding mode (By similarity). Displays dsDNA endonuclease activity that is sequence non-specific (By similarity).

See full target information N

대체 명칭 보기

Nucleoprotein, Nucleocapsid protein, Protein N

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

Indirect ELISA - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

Western blot - Anti-Mouse Crimean-Congo hemorrhagic fever virus Nucleoprotein antibody [A4] - BSA and Azide free (AB324847)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise