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AB326996

Anti-Mouse CYP2E1 antibody [EPR29450-535] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Cytochrome P450 2E1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
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Sandwich ELISA - Anti-Mouse CYP2E1 antibody [EPR29450-535] - BSA and Azide free (Capture) (AB326996)
  • sELISA

Lab

Sandwich ELISA - Anti-Mouse CYP2E1 antibody [EPR29450-535] - BSA and Azide free (Capture) (AB326996)

Interpolated concentration of Mouse CYP2E1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : starting concentration of the first point of recovery in .005 mg/ml dilution of Mouse Liver lysate. The interpolated dilution factor corrected values are plotted (mean +/- standard deviation, n=2). Sample dilutions are made 1% BSA buffer. We do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Mouse CYP2E1 antibody [EPR29450-535] - BSA and Azide free (Capture) (AB326996)
  • sELISA

Lab

Sandwich ELISA - Anti-Mouse CYP2E1 antibody [EPR29450-535] - BSA and Azide free (Capture) (AB326996)

Sandwich ELISA using Mouse CYP2E1 immunogen protein standard curve with the capture antibody ab326996 dilution at 2 µg/mL and detector antibody ab326997 dilution at 0.5 µg/mL. The background-subtracted absorbance data are presented as mean ± standard deviation.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29450-535

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This clone is specific to mouse CYP2E1&#46;</p>

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

제품 세부 정보

ab326996 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse CYP2E1. The recommended pair for sandwich ELISA is:

Capture: ab326996, Mouse CYP2E1 Capture Antibody (unconjugated)

Detector: ab326997, Mouse CYP2E1 Detector Antibody (unconjugated)

The reference range value is 468.75-30,000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
See full target information Cyp2e1

대체 명칭 보기

Cyp2e, Cyp2e-1, Cyp2e1, Cytochrome P450 2E1, 4-nitrophenol 2-hydroxylase, CYPIIE1, Cytochrome P450-ALC, Cytochrome P450-J

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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