Anti-Mouse IgG antibody [RM104]

• ELISA

Supplier Data

ELISA - Anti-Mouse IgG antibody [RM104] (AB190475)

ELISA titre of ab190475 mouse IgG. Plate was coated with 2 - 0.05 μg mouse IgG and a serial dilution of ab190475 (0.2-0.003 μg/ml) used as the primary antibody followed by alkaline phosphatase anti-rabbit IgG.

• ELISA

Supplier Data

ELISA - Anti-Mouse IgG antibody [RM104] (AB190475)

ELISA analysis of ab190475 at 10 - 200 ng/mL using 50 ng/well of different immunoglobulins and an alkaline phosphatase conjugated anti-rabbit IgG.

• WB

CiteAb

Western blot - Anti-Mouse IgG antibody [RM104] (AB190475)

Mouse IgG western blot using anti-Mouse IgG antibody [RM104] ab190475. Publication image and figure legend from Wu, Q., Yuan, X., et al., 2019, Aging (Albany NY), PubMed 31467256.

ab190475 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab190475 please see the product overview.

MiR-181a decelerates pericyte loss and blood-brain barrier breakdown in APP/PS1 mice. (A–D) Lentiviral empty vector or lentiviral miR-181a expressing vector was injected into the hippocampus of APP/PS1 mice aged 5-month-old or 8-month-old. Wild-type age-matched littermates were used as controls. Eight mice were included in each group. One month later, mice were used for subsequent biochemical analyses. (A) The representative immunofluorescent images of CD13-positive pericytes (red) and lectin-positive capillary endothelium (green) in 9-month-old WT and APP/PS1 mice. Scale bar, 100 μm. (B) Quantification of CD13-positive pericytes in the cortex and hippocampus of 6-month-old or 9-month-old WT and APP/PS1 mice. Results represent the number of CD13-positive pericytes per mm2. (C–D) The level of IgG in capillary-depleted cortical extracts from WT mice and 6-month-old or 9-month-old APP/PS1 mice was determined by Western blot analysis. β-actin was used as a loading control. The representative blot images (C) and quantification analysis of IgG level (D) are shown. All data are mean ± SD, and compared by one-way ANOVA followed by Tukey's post-hoc tests. **, p < 0.01.

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