Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] - BSA and Azide free (AB324853)

This data was developed using ab322959, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A virus M1 protein with ab322959 at 1/2000 (0.50 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a Influenza A virus M1 protein expression vector containing a myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab322959 at 1/2000 dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

-ve control 2 : ab9106 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] - BSA and Azide free (AB324853)

This data was developed using ab322959, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322959 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at 1 : 1000 dilution dilution.

Antigen : Influenza A virus M1 protein.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] - BSA and Azide free (AB324853)

This data was developed using ab322959, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Mouse Influenza A virus M1 protein antibody [1G1A12] (<a href='/ko/products/primary-antibodies/mouse-influenza-a-virus-m1-protein-antibody-1g1a12-ab322959'>ab322959</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Influenza A virus M1 protein expression vector containi a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 28 kDa,124 kDa,20-28 kDa

false

Exposure time: 1s