AB250121

Anti-MRPL30 antibody [EPR12502] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal MRPL30 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

MRPL28, RPML28, HSPC249, MRPL30, Large ribosomal subunit protein uL30m, L28mt, MRP-L28, L30mt, MRP-L30

1 이미지

Supplier Data

Western blot - Anti-MRPL30 antibody [EPR12502] - BSA and Azide free (AB250121)

This data was developed using ab179819, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MRPL30 antibody [EPR12502] (<a href='/ko/products/primary-antibodies/mrpl30-antibody-epr12502-ab179819'>ab179819</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 19 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12502

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab250121 is the carrier-free version of ab179819.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MRPL30 also known as Mitochondrial Ribosomal Protein L30 is a component of the mitochondrial ribosome. This protein has a molecular mass of approximately 42.7 kDa and is encoded by the MRPL30 gene. Expression of MRPL30 occurs mainly in mitochondria across various tissues where it is involved in protein synthesis within the mitochondria. Its presence is important for the maintenance of mitochondrial function as it helps translate mitochondrial RNA into essential proteins.

Biological function summary

MRPL30 contributes to mitochondrial protein synthesis as a part of the large subunit in the mitochondrial ribosome complex. This complex differs from cytoplasmic ribosomes due to its unique protein-to-rRNA composition reflecting its specific role within mitochondria. MRPL30's involvement in the formation of the ribosomal subunit supports the production of proteins necessary for oxidative phosphorylation. This synthesis is essential for energy production and normal cellular metabolism.

Pathways

The functioning of MRPL30 integrates into mitochondrial protein synthesis and oxidative phosphorylation pathways. The protein ensures the efficient translation of mitochondrial-encoded genes important for ATP production through oxidative phosphorylation. It interacts with other ribosomal proteins such as MRPL28 and MRPL44 important for the assembly and function of the mitochondrial ribosome and thereby sustaining cellular energy demands.

Alterations in MRPL30 expression or function can link to mitochondrial dysfunction-related disorders including mitochondrial diseases. These diseases often result from impaired ATP production due to defective oxidative phosphorylation. Research also associates MRPL30 with neurodegenerative diseases as mitochondria play an important role in neuronal function. Its interaction with other mitochondrial ribosomal proteins like MRPS12 can affect mitochondrial translation stability contributing to the pathophysiology of such disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information MRPL30

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MRPL30 antibody [EPR12502] - BSA and Azide free

Western blot - Anti-MRPL30 antibody [EPR12502] - BSA and Azide free (AB250121)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise