Rabbit Polyclonal MS2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADAM8 aa 650 to C-terminus.
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CD156a, MS2, ADAM8, Disintegrin and metalloproteinase domain-containing protein 8, ADAM 8, Cell surface antigen MS2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody (AB236949)
Paraffin-embedded human pancreas tissue stained for MS2 using ab236949 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody (AB236949)
Paraffin-embedded human lymph node tissue stained for MS2 using ab236949 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MS2 antibody (AB236949)
MCF7 (human breast adenocarcinoma cell line) cells stained for MS2 (green) using ab236949 at 1/133 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).
Reactivity 정보
특성 및 보관 정보
제형
Purification 테크닉
Purification 관련 사항
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
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제품이 사용된 논문 (2)
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iScience 28:112748 PubMed40585507
2025
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Cellular & molecular biology letters 29:75 PubMed38755530
2024
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