JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236949

Anti-MS2 antibody

Be the first to review this product! 리뷰 제출

|

(2 제품이 사용된 논문 )

Rabbit Polyclonal MS2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADAM8 aa 650 to C-terminus.

대체 명칭 보기

CD156a, MS2, ADAM8, Disintegrin and metalloproteinase domain-containing protein 8, ADAM 8, Cell surface antigen MS2

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody (AB236949)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody (AB236949)

Paraffin-embedded human pancreas tissue stained for MS2 using ab236949 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody (AB236949)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MS2 antibody (AB236949)

Paraffin-embedded human lymph node tissue stained for MS2 using ab236949 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-MS2 antibody (AB236949)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MS2 antibody (AB236949)

MCF7 (human breast adenocarcinoma cell line) cells stained for MS2 (green) using ab236949 at 1/133 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI (Blue).

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ADAM8 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P78325

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Possible involvement in extravasation of leukocytes.
See full target information ADAM8

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 28:112748 PubMed40585507

2025

CETSA-MS unveils novel targets engaged by rigosertib to promote anti-tumor activity and inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Petros Kechagioglou,Hajime Yurugi,Camille Dupont,Alexey Chernobrovkin,Rossana Romero,Gregory Harms,Marie Oster,Sandra Ciesek,Rebecca Tweedell,Thirumala-Devi Kanneganti,Stefanie Zimmer,Stephen Cosenza,Steven M Fruchtman,Krishnaraj Rajalingam

Cellular & molecular biology letters 29:75 PubMed38755530

2024

Blockade of the ADAM8-Fra-1 complex attenuates neuroinflammation by suppressing the Map3k4/MAPKs axis after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanyang Qian,Rulin Li,Tianyu Zhao,Kunxin Xie,PengFei Li,Guangshen Li,Na Shen,Jiamin Gong,Xin Hong,Lei Yang,Haijun Li
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com