Anti-MSRA antibody [EPR29159-5]

• WB

Supplier Data

Western blot - Anti-MSRA antibody [EPR29159-5] (AB322706)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HEK-293 (PMID : 32482536), B16-F10.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706) at 1/1000 dilution

Lane 1:

NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 4:

B16-F10 (mouse skin melanoma cell) whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,36 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MSRA antibody [EPR29159-5] (AB322706)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Human colon, thyroid, brainstem, and fat; Mouse skeletal muscle (PMID : 19769460) and rat skeletal muscle.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-5 : 114 seconds; Lane 6-9 : 15 seconds

All lanes:

Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Lane 3:

Human thyroid tissue lysate at 20 µg

Lane 4:

Human brainstem tissue lysate at 20 µg

Lane 5:

Human fat tissue lysate at 20 µg

Lane 6:

Mouse kidney tissue lysate at 20 µg

Lane 7:

Mouse skeletal tissue lysate at 20 µg

Lane 8:

Rat kidney tissue lysate at 20 µg

Lane 9:

Rat skeletal tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,36 kDa

true