Anti-MTAP antibody [MTAP/1813]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [MTAP/1813] (AB238024)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MTAP using ab238024 at 2 μg/ml in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-MTAP antibody [MTAP/1813] (AB238024)

Western blot : Anti-MTAP antibody [MTAP/1813] ab238024 staining at 1 µg/mL, shown in green; Rabbit anti alpha Tubulin ab52866 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 31kDa in Wild-type HeLa cell lysates with no signal observed at this size in MTAP knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-MTAP antibody [MTAP/1813] (ab238024) at 1 µg/mL

Lane 1:

Wild-type HeLa cell lysates at 20 µg

Lane 2:

MTAP knockout HeLa cell lysates at 20 µg

Lane 3:

HepG2 cell lysates at 20 µg

Lane 4:

A549 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

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• Protein Array

Unknown

Protein Array - Anti-MTAP antibody [MTAP/1813] (AB238024)

ab238024 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

• WB

Supplier Data

Western blot - Anti-MTAP antibody [MTAP/1813] (AB238024)

All lanes:

Western blot - Anti-MTAP antibody [MTAP/1813] (ab238024) at 2 µg/mL

All lanes:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 31 kDa

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