AB243815

Anti-MTFMT/FMT antibody

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Rabbit Polyclonal MTFMT/FMT antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MTFMT aa 50-200.

대체 명칭 보기

FMT, FMT1, MTFMT, MtFMT

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-MTFMT/FMT antibody (AB243815)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTFMT/FMT antibody (AB243815)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling MTFMT/FMT using ab243815 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTFMT/FMT antibody (AB243815)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTFMT/FMT antibody (AB243815)

Formalin-fixed, paraffin-embedded human stomach tissue stained for MTFMT/FMT with ab243815 at a 1/20 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MTFMT aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q96DP5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MTFMT also known as mitochondrial methionyl-tRNA formyltransferase or FMT is an enzyme that plays an important role in protein translation within mitochondria. It adds a formyl group to the initiator methionyl-tRNA which is essential for protein synthesis. MTFMT weighs about 43 kDa and is predominantly expressed in mitochondrial regions of human cells. This enzyme functions in concert with other mitochondrial proteins to ensure efficient initiation of protein translation.

Biological function summary

The enzyme contributes significantly to the process of mitochondrial protein biosynthesis. By formylating methionyl-tRNA MTFMT facilitates the correct start of translation which is critical for mitochondrial function. It does not seem to form part of larger protein complexes but it interacts with other translation machinery proteins to support mitochondrial function effectively. Mitochondria being powerhouses of cells rely on such proteins for the generation of energy in the form of ATP.

Pathways

Methionine formylation by MTFMT integrates into the broader process of mitochondrial translation initiation. This process plays a part in the oxidative phosphorylation pathway a central component of cellular respiration. Within this pathway MTFMT's mechanism is linked to the assembly and function of the electron transport chain. Proteins like MT-ND1 and COX5A which reside in the electron transport chain rely indirectly on MTFMT's action for proper function and assembly of mitochondrial complexes.

Mutations or deficiencies in MTFMT can disrupt mitochondrial protein synthesis leading to mitochondrial diseases. One condition associated with MTFMT malfunction is Leigh syndrome a severe neurological disorder that affects energy production. Such diseases often display overlaps in symptoms linked not just to MTFMT but also to other mitochondrial-related proteins like SURF1 and COX10. These associations underline the critical role of MTFMT in maintaining mitochondrial integrity and cellular energy balance.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Methionyl-tRNA formyltransferase that formylates methionyl-tRNA in mitochondria and is crucial for translation initiation.

See full target information MTFMT

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Investigative ophthalmology & visual science 66:24 PubMed40402518

2025

SOX4 as a Key Oncogene Driving Tumor Invasion in Retinoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahe Nie,Junjie Tang,Zhihui Zhang,Hetian Sun,Xinyue Wang,Longxiang Wang,Meng Wang,Weifeng Huang,Yang Gao,Jinmiao Li,Chao Cheng,Shicai Su,Shuxia Chen,Siming Ai,Yuxiang Mao,Ping Zhang,Yaoming Liu,Rong Lu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTFMT/FMT antibody

Immunocytochemistry/ Immunofluorescence - Anti-MTFMT/FMT antibody (AB243815)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTFMT/FMT antibody (AB243815)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Secondary Antibodies

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise