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AB236737

Anti-MTHFR antibody

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Rabbit Polyclonal MTHFR antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MTHFR aa 1 to C-terminus.

대체 명칭 보기

Methylenetetrahydrofolate reductase (NADPH), MTHFR

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-MTHFR antibody (AB236737)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTHFR antibody (AB236737)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for MTHFR (green) using ab236737 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFR antibody (AB236737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFR antibody (AB236737)

Paraffin-embedded human prostate cancer tissue stained for MTHFR using ab236737 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MTHFR aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P42898

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대체품

Primary Antibodies

AB203786

Anti-MTHFR antibody [EPR19781]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MTHFR antibody [EPR19781] (AB203786)

  • 0
  • 0

보완 제품

Primary Antibodies

AB66039

Anti-MTR antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-MTR antibody (AB66039)

  • 4
  • 1
Primary Antibodies

AB226341

Anti-MTHFD1 antibody

Immunoprecipitation - Anti-MTHFD1 antibody (AB226341)

  • 0
  • 0
Primary Antibodies

AB140600

Anti-CBS antibody [EPR8579]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBS antibody [EPR8579] (AB140600)

  • 5
  • 1
Primary Antibodies

AB316329

Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free

BOND RX™ Validated|Recombinant|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody [EPR28607-51] - BSA and Azide free (AB316329)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 'MTHFR' protein also known as methylenetetrahydrofolate reductase plays an important role in folate metabolism by catalyzing the reduction of 510-methylenetetrahydrofolate to 5-methyltetrahydrofolate. This enzyme has a mass of approximately 70 kDa and is expressed in various tissues including liver kidney and brain. It exists primarily in the cytoplasm where it performs its enzymatic functions critical for cellular metabolism.
Biological function summary

Methylenetetrahydrofolate reductase is essential in the process of transferring one-carbon units especially in the synthesis of the amino acid methionine and the methylation of DNA. This enzyme does not form part of a larger protein complex but operates independently in the folate cycle. Its activity directly influences homocysteine levels within the cell with alterations in its function affecting many metabolic processes.

Pathways

Methylenetetrahydrofolate reductase is an important component of the folate and methionine cycles significant in the remethylation of homocysteine to methionine. These pathways intersect with transmethylation and nucleotide synthesis essential for cell functions and replication. It interacts with proteins like methionine synthase and cystathionine beta-synthase which further demonstrate the enzyme's importance in maintaining cellular homeostasis.

Methylenetetrahydrofolate reductase deficiency or mutations often lead to hyperhomocysteinemia and have been associated with cardiovascular diseases and neurological disorders. Mutations in this enzyme can alter its efficiency leading to the accumulation of homocysteine. This has connections with proteins such as methionine synthase where impaired functioning due to MTHFR mutation can exacerbate the disruption of homocysteine metabolism contributing to disease states.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine (PubMed : 29891918). Represents a key regulatory connection between the folate and methionine cycles (Probable).
See full target information MTHFR
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 28:112974 PubMed40687831

2025

Mutation T9I in Envelope confers autophagy resistance to SARS-CoV-2 Omicron.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Klute,Rayhane Nchioua,Arne Cordsmeier,Jyoti Vishwakarma,Lennart Koepke,Hala Alshammary,Christoph Jung,Maximilian Hirschenberger,Helene Hoenigsperger,Jana-Romana Fischer,Rinu Sivarajan,Fabian Zech,Steffen Stenger,Ruth Serra-Moreno,Ana Silvia Gonzalez-Reiche,Emilia Mia Sordillo,Harm van Bakel,Viviana Simon,Frank Kirchhoff,Timo Jacob,Dorota Kmiec,Andreas Pichlmair,Armin Ensser,Konstantin Maria Johannes Sparrer
제품이 사용된 논문 모두 보기
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Product promise

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