AB223719

Anti-MTM1 antibody

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Rabbit Polyclonal MTM1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MTM1 aa 150-300.

대체 명칭 보기

CG2, MTM1, Myotubularin, Phosphatidylinositol-3-phosphate phosphatase

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTM1 antibody (AB223719)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTM1 antibody (AB223719)

Paraffin-embedded human kidney tissue stained for MTM1 using ab223719 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MTM1 antibody (AB223719)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MTM1 antibody (AB223719)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for MTM1 (green) using ab223719 at 4 μg/ml in ICC/IF.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MTM1 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q13496

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MTM1 also known as myotubularin is a phosphatase important for lipid metabolism specifically phosphoinositide signaling. The protein has a mass of approximately 69 kDa. MTM1 is expressed in skeletal muscle tissues and plays an important mechanical role in muscle maintenance. Its expression ensures proper muscle function and growth by regulating the levels of phosphatidylinositol 3-phosphate (PI3P) within cellular membranes.

Biological function summary

MTM1 is important for muscle cell maintenance and development. The protein is a part of a larger complex that includes other myotubularin-related proteins providing a coordinated regulation of phosphoinositide metabolism. MTM1 ensures the stability and elongation of muscle fibers contributing to normal muscle physiology by facilitating cellular signaling pathways that are necessary for muscle integrity and survival.

Pathways

MTM1 plays an essential role in the phosphoinositide signaling and PI3K/AKT pathways. These pathways are significant for cell growth differentiation and survival. MTM1 interacts with proteins pivotal to these pathways such as AKT and mTOR to regulate cellular processes linked with energy balance and response to growth signals. Without proper MTM1 function these pathways may become dysregulated leading to muscle-related issues.

MTM1 mutations directly associate with X-linked myotubular myopathy a severe muscle disorder. The failure in MTM1 function causes muscle weakness and structural abnormalities due to impaired phosphoinositide metabolism. This myopathy highlights MTM1's interaction with dynamin 2 another protein altered in related muscle diseases which further demonstrates MTM1’s critical role in maintaining muscle function and health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Lipid phosphatase which dephosphorylates phosphatidylinositol 3-monophosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) (PubMed : 10900271, PubMed : 11001925, PubMed : 12646134, PubMed : 14722070). Has also been shown to dephosphorylate phosphotyrosine- and phosphoserine-containing peptides (PubMed : 9537414). Negatively regulates EGFR degradation through regulation of EGFR trafficking from the late endosome to the lysosome (PubMed : 14722070). Plays a role in vacuolar formation and morphology. Regulates desmin intermediate filament assembly and architecture (PubMed : 21135508). Plays a role in mitochondrial morphology and positioning (PubMed : 21135508). Required for skeletal muscle maintenance but not for myogenesis (PubMed : 21135508). In skeletal muscles, stabilizes MTMR12 protein levels (PubMed : 23818870).

See full target information MTM1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 9: PubMed39315548

2024

Pyruvate metabolism dictates fibroblast sensitivity to GLS1 inhibition during fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Greg Contento,Jo-Anne Am Wilson,Brintha Selvarajah,Manuela Platé,Delphine Guillotin,Valle Morales,Marcello Trevisani,Vanessa Pitozzi,Katiuscia Bianchi,Rachel C Chambers

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTM1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTM1 antibody (AB223719)

Immunocytochemistry/ Immunofluorescence - Anti-MTM1 antibody (AB223719)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise