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AB240569

Anti-MTMR1 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal MTMR1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MTMR1 aa 600-700.

대체 명칭 보기

Phosphatidylinositol-3-phosphate phosphatase MTMR1, Myotubularin-related protein 1, MTMR1

2 이미지
Immunoprecipitation - Anti-MTMR1 antibody (AB240569)
  • IP

Supplier Data

Immunoprecipitation - Anti-MTMR1 antibody (AB240569)

MTMR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. For western blotting, ab240569 was used at 0.4 μg/ml.

Lane 1 : ab240569 used for IP at 6 μg per reaction.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-MTMR1 antibody (ab240569)

Predicted band size: 75 kDa

false

Western blot - Anti-MTMR1 antibody (AB240569)
  • WB

Supplier Data

Western blot - Anti-MTMR1 antibody (AB240569)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-MTMR1 antibody (ab240569) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 75 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human MTMR1 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

Q13613

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보완 제품

Primary Antibodies

AB222206

Anti-FAN1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FAN1 antibody (AB222206)

  • 0
  • 0
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85
Primary Antibodies

AB11575

Anti-Laminin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

  • 5
  • 74
Primary Antibodies

AB9110

Anti-HA tag antibody - ChIP Grade

Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (AB9110)

  • 5
  • 59
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab240569 was affinity purified using an epitope specific to MTMR1 immobilized on solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MTMR1 also known as Myotubularin-related protein 1 belongs to the myotubularin family of phosphatidylinositol (PI) phosphatases. It plays a role in regulating phosphoinositide levels. The protein has a molecular mass of approximately 72 kDa. Scientists have found its expression in various tissues including skeletal muscle and the nervous system. MTMR1 is important for cellular homeostasis by modulating lipid signaling pathways.
Biological function summary

The protein functions as a phosphatase that removes phosphate groups from phosphatidylinositol 3-phosphate and phosphatidylinositol 35-bisphosphate. It does not work alone but as part of complexes that include other proteins from the same family. This collaboration allows for regulating PI levels which are involved in membrane trafficking and signal transduction. Its activity impacts cellular processes like endocytosis and autophagy which are important for cell maintenance and survival.

Pathways

MTMR1 is involved in pathways that include lipid signaling and autophagy. It particularly affects the PI3K-Akt signaling pathway which is important for controlling cell growth and apoptosis. MTMR1 can interact with other members of the myotubularin family like MTMR2 to execute its functions in these pathways. The interconnected work of these proteins ensures the correct functioning of signal transduction and cellular homeostasis.

MTMR1 has connections with muscular and neurological diseases. Particularly its dysfunction has been noted in types of muscular dystrophy. Researchers have linked its activity with proteins such as MTMR13 showing combined roles in disease mechanisms especially those affecting muscle tissue. Studies continue to explore these associations to understand better and manage related disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Lipid phosphatase that specifically dephosphorylates the D-3 position of phosphatidylinositol 3-phosphate, generating phosphatidylinositol (PubMed : 11733541, PubMed : 27018598). Could also dephosphorylate phosphatidylinositol 3,5-bisphosphate to produce phosphatidylinositol 5-phosphate (PubMed : 27018598).
See full target information MTMR1
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature 637:983-990 PubMed39663456

2024

In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor.

Applications

Unspecified application

Species

Unspecified reactive species

Huaipeng Xing,Remus R E Rosenkranz,Piere Rodriguez-Aliaga,Ting-Ting Lee,Tomáš Majtner,Stefanie Böhm,Beata Turoňová,Judith Frydman,Martin Beck

Cell reports 33:108264 PubMed33053349

2020

Activation of the CARD8 Inflammasome Requires a Disordered Region.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley J Chui,Andrew R Griswold,Cornelius Y Taabazuing,Elizabeth L Orth,Kuo Gai,Sahana D Rao,Daniel P Ball,Jeffrey C Hsiao,Daniel A Bachovchin
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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