AB323645

Anti-Mu Opioid Receptor antibody [EPR29112-126]

BOND RX™ Validated
Advanced Validation
RabMAb
Recombinant
20ul selling size
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Rabbit Recombinant Monoclonal Mu Opioid Receptor antibody. Suitable for Dot, IHC-P, mIHC and reacts with Transfected cell lysate - Mouse, Mouse, Rat samples.

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Multiplex immunohistochemistry - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue staining Mu Opioid Receptor with ab323645 at a 1 : 2000 (0.252 ug/ml) dilution, ab307138 anti-TAC1 used at 1 : 500 (0.966 ug/ml) dilution and ab316105 anti-GPCR GPR17 used at a 1 : 500 (0.509ug/ml) dilution.

Panel A : merged staining of anti-OPRM1 (magenta; Opal™520), anti-TAC1 (green; Opal™690) and anti-GPCR GPR17 (yellow; Opal™570) on mouse spinal cord.
Panel B : anti-OPRM1 showed positive staining in mouse spinal cord.
Panel C : anti-TAC1 showed positive staining in mouse spinal cord.
Panel D : anti-GPCR GPR17 staining oligodendrocytes in mouse spinal cord.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323645, ab307138 and ab316105 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Mu Opioid Receptor with ab323645 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat liver (PMID : 7605935). The section was incubated with ab323645 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mu Opioid Receptor with ab323645 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse liver. The section was incubated with ab323645 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Mu Opioid Receptor with ab323645 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse spleen. The section was incubated with ab323645 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Mu Opioid Receptor with ab323645 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebrum. The section was incubated with ab323645 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemical analysis of paraffin-embedded Rat spinal cord tissue labeling Mu Opioid Receptor with ab323645 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat spinal cord (PMID : 32723769; PMID : 29473323). The section was incubated with ab323645 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue staining Mu Opioid Receptor with ab323645 at a 1 : 2000 (0.252 ug/ml) dilution, ab307138 anti-TAC1 used at 1 : 500 (0.966 ug/ml) dilution and ab316105 anti-GPCR GPR17 used at a 1 : 500 (0.509ug/ml) dilution.

Panel A : merged staining of anti-OPRM1 (magenta; Opal™520), anti-TAC1 (green; Opal™690) and anti-GPCR GPR17 (yellow; Opal™570) on rat spinal cord.
Panel B : anti-OPRM1 showed positive staining in rat spinal cord.
Panel C : anti-TAC1 showed positive staining in rat spinal cord.
Panel D : anti-GPCR GPR17 staining oligodendrocytes in rat spinal cord.
Nuclear DNA was labeled with DAPI (shown in blue).

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Mu Opioid Receptor with ab323645 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum (PMID : 11717463). The section was incubated with ab323645 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Mu Opioid Receptor with ab323645 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat spleen. The section was incubated with ab323645 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labeling Mu Opioid Receptor with ab323645 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spinal cord (PMID : 11717463). The section was incubated with ab323645 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Dot Blot - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Dot blot analysis of Mu Opioid Receptor using ab323645 at 1 : 1000 (0.504 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : 293T cells transfected with a mouse Mu Opioid Receptor expression vector containing a His-tag, whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-Mu Opioid Receptor antibody [EPR29112-126] (ab323645) at 1/1000 dilution

All lanes:

293T cells transfected with a mouse Mu Opioid Receptor expression vector containing a His-tag, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29112-126

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

mIHC, IHC-P, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/2000", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/2000", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Mouse": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor for endogenous opioids such as beta-endorphin and endomorphin (PubMed : 10842167, PubMed : 16682964, PubMed : 21422164, PubMed : 22437502, PubMed : 26245379, PubMed : 7797593, PubMed : 9037090). Receptor for natural and synthetic opioids including morphine, heroin, DAMGO, fentanyl, etorphine, buprenorphin and methadone (PubMed : 16682964, PubMed : 7797593, PubMed : 9037090). Also activated by enkephalin peptides, such as Met-enkephalin or Met-enkephalin-Arg-Phe, with higher affinity for Met-enkephalin-Arg-Phe (PubMed : 35201898, PubMed : 6933569). Agonist binding to the receptor induces coupling to an inactive GDP-bound heterotrimeric G-protein complex and subsequent exchange of GDP for GTP in the G-protein alpha subunit leading to dissociation of the G-protein complex with the free GTP-bound G-protein alpha and the G-protein beta-gamma dimer activating downstream cellular effectors (PubMed : 10842167, PubMed : 21422164, PubMed : 22437502). The agonist- and cell type-specific activity is predominantly coupled to pertussis toxin-sensitive G(i) and G(o) G alpha proteins, GNAI1, GNAI2, GNAI3 and GNAO1 isoforms Alpha-1 and Alpha-2, and to a lesser extent to pertussis toxin-insensitive G alpha proteins GNAZ and GNA15 (PubMed : 26245379, PubMed : 9767386). They mediate an array of downstream cellular responses, including inhibition of adenylate cyclase activity and both N-type and L-type calcium channels, activation of inward rectifying potassium channels, mitogen-activated protein kinase (MAPK), phospholipase C (PLC), phosphoinositide/protein kinase (PKC), phosphoinositide 3-kinase (PI3K) and regulation of NF-kappa-B (By similarity). Also couples to adenylate cyclase stimulatory G alpha proteins (By similarity). The selective temporal coupling to G-proteins and subsequent signaling can be regulated by RGSZ proteins, such as RGS9, RGS17 and RGS4 (PubMed : 15827571, PubMed : 17725581). Phosphorylation by members of the GPRK subfamily of Ser/Thr protein kinases and association with beta-arrestins is involved in short-term receptor desensitization (By similarity). Beta-arrestins associate with the GPRK-phosphorylated receptor and uncouple it from the G-protein thus terminating signal transduction (By similarity). The phosphorylated receptor is internalized through endocytosis via clathrin-coated pits which involves beta-arrestins (PubMed : 12642578). The activation of the ERK pathway occurs either in a G-protein-dependent or a beta-arrestin-dependent manner and is regulated by agonist-specific receptor phosphorylation (By similarity). Acts as a class A G-protein coupled receptor (GPCR) which dissociates from beta-arrestin at or near the plasma membrane and undergoes rapid recycling (By similarity). Receptor down-regulation pathways are varying with the agonist and occur dependent or independent of G-protein coupling. Endogenous ligands induce rapid desensitization, endocytosis and recycling (By similarity). Heterooligomerization with other GPCRs can modulate agonist binding, signaling and trafficking properties (By similarity).. Isoform 9. Isoform 9 is involved in morphine-induced scratching and seems to cross-activate GRPR in response to morphine.

See full target information Oprm1

Additional targets

Oprm1

대체 명칭 보기

Mor, Oprm, Mu-type opioid receptor, M-OR-1, MOR-1, Ror-b, Oprm1, Mu-type opioid receptor, M-OR-1, MOR-1, Opioid receptor B

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mu Opioid Receptor antibody [EPR29112-126]

Multiplex immunohistochemistry - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Multiplex immunohistochemistry - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

Dot Blot - Anti-Mu Opioid Receptor antibody [EPR29112-126] (AB323645)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

Product promise