JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB232420

Anti-MUC4 antibody [EPR9308] - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal MUC4 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR9308] - BSA and Azide free (AB232420)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR9308] - BSA and Azide free (AB232420)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling MUC4 with purified ab150381 at 1 : 750 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150381).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR9308] - BSA and Azide free (AB232420)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR9308] - BSA and Azide free (AB232420)

Immunohistochemical analysis of paraffin embedded Human Stomach adenocarcinoma tissue using unpurified ab150381 showing -ve staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150381).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR9308] - BSA and Azide free (AB232420)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR9308] - BSA and Azide free (AB232420)

Immunohistochemical analysis of paraffin embedded Human Pancreatic carcinoma tissue using unpurified ab150381 showing +ve staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150381).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR9308] - BSA and Azide free (AB232420)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR9308] - BSA and Azide free (AB232420)

Immunohistochemical analysis of paraffin embedded normal Human pancreas tissue using unpurified ab150381 showing -ve staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150381).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9308

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

대체품

Primary Antibodies

AB60720

Anti-MUC4 antibody [5B12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [5B12] (AB60720)

  • 4
  • 2
Primary Antibodies

AB263957

Anti-MUC4 antibody [3C7.D8.E11.F5]

Western blot - Anti-MUC4 antibody [3C7.D8.E11.F5] (AB263957)

  • 0
  • 0
Primary Antibodies

AB214220

Anti-MUC4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody (AB214220)

  • 0
  • 0
Primary Antibodies

AB183320

Anti-MUC4 antibody [SP241] - C-terminal

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [SP241] - C-terminal (AB183320)

  • 0
  • 0
Primary Antibodies

AB267547

Alexa Fluor® 647 Anti-MUC4 antibody [SP241]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-MUC4 antibody [SP241] (AB267547)

  • 0
  • 0
Primary Antibodies

AB243921

Anti-MUC4 antibody [SP241] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [SP241] - BSA and Azide free (AB243921)

  • 0
  • 0
Primary Antibodies

AB267546

Alexa Fluor® 488 Anti-MUC4 antibody [SP241]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-MUC4 antibody [SP241] (AB267546)

  • 0
  • 0

보완 제품

Sample Prep & Detection Kits

AB201806

DyLight® 800 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - DyLight® 800 Conjugation Kit (Fast) - Lightning-Link® (AB201806)

  • 0
  • 0
Sample Prep & Detection Kits

AB154876

Gold Conjugation Kit (80nm, 20 OD)

Conjugation - Gold Conjugation Kit (80nm, 20 OD) (AB154876)

  • 0
  • 0
Primary Antibodies

AB110641

Anti-BRG1 antibody [EPNCIR111A]

20ul selling size|BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

ChIC/CUT&RUN sequencing - Anti-BRG1 antibody [EPNCIR111A] (AB110641)

  • 5
  • 10
Primary Antibodies

AB223846

Anti-Gastric Mucin/MUC-6 antibody [EPR20623]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gastric Mucin/MUC-6 antibody [EPR20623] (AB223846)

  • 4
  • 2
Primary Antibodies

AB109185

Anti-MUC1 antibody [EPR1023]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC1 antibody [EPR1023] (AB109185)

  • 0
  • 1
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
style
left-column

제품 세부 정보

ab232420 is the carrier-free version of ab150381.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
style
left-column
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed : 10880978). These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed : 10880978). Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.
See full target information MUC4

대체 명칭 보기

Mucin-4, MUC-4, Ascites sialoglycoprotein, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, ASGP, MUC4
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com