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AB9879

Anti-Mumps nucleoprotein antibody [5E3]

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(1 출판물)

Mouse Monoclonal NCAP antibody. Suitable for I-ELISA, WB and reacts with Mumps orthorubulavirus, Recombinant full length protein - Mumps orthorubulavirus samples. Cited in 1 publication.
1 이미지
Western blot - Anti-Mumps nucleoprotein antibody [5E3] (AB9879)
  • WB

Unknown

Western blot - Anti-Mumps nucleoprotein antibody [5E3] (AB9879)

Western blot of yeast-derived mumps NP using ab9879 at a concentration of 0.5 μg/ml. Western blot of yeast-derived mumps NP using ab9879 at a concentration of 0.5 µg/ml.

All lanes:

Western blot - Anti-Mumps nucleoprotein antibody [5E3] (ab9879)

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

5E3

Isotype

IgG2a

Light chain 타입

unknown

Carrier free

No

Reacts with

Mumps orthorubulavirus

Applications

I-ELISA, WB

applications

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mumps orthorubulavirus": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "1/1000 - 1/4997", "IELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Mumps orthorubulavirus": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }

제품 세부 정보

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The mumps nucleoprotein sometimes referred to as N protein plays an important role in the replication and transcription of the mumps virus acting as a structural element in the formation of viral ribonucleoprotein (RNP) complexes. The nucleoprotein is a large protein with a molecular mass of approximately 60 kDa and is expressed in cells that are infected by the mumps virus. It facilitates encapsidation of viral RNA and ensures the stability of the viral genome which is essential for virus survival and propagation.
Biological function summary

The mumps nucleoprotein functions as a part of the mumps virus replication complex. It interacts with other viral proteins such as the phosphoprotein (P protein) and the large protein (L protein) to initiate and regulate the replication of viral RNA. These interactions are critical for the stabilization of the entire complex ensuring the efficient production of new viral particles within host cells. The nucleoprotein is primarily located in the cytoplasm of infected cells where it associates with newly synthesized viral RNA strands.

Pathways

The mumps nucleoprotein is involved in the viral replication and transcription pathways specific to the mumps virus. These pathways facilitate viral spread and infection contributing to the virulence of the virus. The nucleoprotein works closely with L and P proteins which serve vital roles in the RNA polymerase complex guiding the synthesis of viral RNA. These interactions are part of the broader negative-strand RNA virus replication pathway shared by other viruses in the Paramyxoviridae family.

The mumps nucleoprotein is directly associated with the mumps disease a contagious viral infection that can lead to severe complications such as encephalitis and orchitis. Antibodies targeting the nucleoprotein can help in diagnosing mumps infections. The nucleoprotein also has implications in mumps-related complications where it interacts with immune system components. Research into this protein helps in understanding the immunological response to the mumps virus potentially informing vaccine development and antiviral therapies.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

See full target information Mumps nucleoprotein

대체 명칭 보기

NP, Nucleocapsid protein, Nucleoprotein

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of medical virology 66:123-30 PubMed11748668

2001

High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology.

Applications

Unspecified application

Species

Unspecified reactive species

Dhanraj Samuel,Kestutis Sasnauskas,Li Jin,Stuart Beard,Aurelija Zvirbliene,Alma Gedvilaite,Bernard Cohen
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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