AB216641

Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody

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Rabbit Polyclonal Muscarinic Acetylcholine Receptor 2/CM2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CHRM2 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

Muscarinic acetylcholine receptor M2, CHRM2

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody (AB216641)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody (AB216641)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human brain glioma tissue labeling Muscarinic Acetylcholine Receptor 2/CM2 with ab216641 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Lab

Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody (AB216641)

All lanes:

Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody (ab216641) at 1/500 dilution

All lanes:

Mouse brain lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/10000 dilution

Predicted band size: 52 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CHRM2 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P08172

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Muscarinic Acetylcholine Receptor 2 (M2) also known as CHRM2 is a G protein-coupled receptor (GPCR) with a molecular mass of about 66 kDa. This receptor is predominantly expressed in cardiac tissue particularly in the atria and plays a fundamental role in the parasympathetic nervous system by mediating the effects of acetylcholine. M2 receptors participate in negative feedback control by inhibiting adenylate cyclase which reduces the conversion of ATP to cAMP affecting heart rate and contractility. These receptors are an important target for anti-muscarinic agents like otenzepad and AF-DX 116 which inhibit the receptor's activity.

Biological function summary

Muscarinic acetylcholine receptors perform diverse functions participating in the regulation of autonomic nervous system responses. M2 receptors are not part of a large protein complex but function closely with other GPCRs to maintain homeostasis. They modulate the activity of various ion channels contributing to their role in cardiac physiological processes. Anti-muscarinic antibodies can specifically target M2 for therapeutic interventions which further helps in dissecting their biological functionality.

Pathways

M2 receptors play essential roles in both the autonomic nervous system and the cardiovascular system. They are involved in the cholinergic signaling pathway which modulates heart rate by influencing ion channel activity and reducing intracellular cAMP levels. Their interaction with proteins like G-proteins and ion channels such as Kir3.1 underlines their importance in these pathways. M2 muscarinic receptors are closely linked with other muscarinic receptors showing a collaborative action in signaling cascades.

M2 receptors have connections with cardiovascular diseases and certain neurological disorders. Alterations in M2 receptor function or expression can lead to conditions like bradycardia where the heart rate is abnormally slow and are also implicated in cognitive disorders linked to cholinergic dysfunction. The receptor's interaction with other muscarinic receptors like M1 and M4 further ties it to these pathologies amplifying its relevance in understanding complex diseases. Anti-muscarinic antibodies targeting M2 can modulate these interactions offering potential therapeutic benefits.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Muscarinic receptor for acetylcholine, a neurotransmitter found in the brain, neuromuscular junctions and the autonomic ganglia (PubMed : 24256733, PubMed : 3443095, PubMed : 36690613). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed : 36690613). CHRM2 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G proteins and mediates signaling by inhibiting adenylate cyclase activity (PubMed : 36690613).

See full target information CHRM2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 21:817 PubMed37974228

2023

HOXD9/miR-451a/PSMB8 axis is implicated in the regulation of cell proliferation and metastasis via PI3K/AKT signaling pathway in human anaplastic thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhong,Fan Yu,Ling Yang,Yu Wang,Lin Liu,Chengyou Jia,Haidong Cai,Jianshe Yang,Shiyang Sheng,Zhongwei Lv,Li Weng,Bo Wu,Xiaoping Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody (AB216641)

Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody (AB216641)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise