Anti-MYBPC3 antibody [EPR25673-58]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cardiac muscle (PMID : 26109061).The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the human skeletal muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat cardiac muscle tissue staining Connexin 43 / GJA1 with ab235585 at 0.507 μg/ml, ab308619 anti-MYBPC3, and ab218198 anti-Dystrophin.

Panel A : anti-Connexin 43 / GJA1 (green; Opal™520), anti-MYBPC3 (magenta; Opal™570), anti-Dystrophin (gray; Opal™690) on rat cardiac muscle.

Panel B : anti-Connexin 43 / GJA1 staining intercalated discs in rat cardiac muscle.

Panel C : anti-MYBPC3 staining cytoplasm of heart myocytes in rat cardiac muscle.

Panel D : anti-Dystrophin staining membrane of heart myocytes in rat cardiac muscle.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab235585, ab308619 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cardiac muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cardiac muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the mouse skeletal muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labelling MYBPC3 with ab308619 at 1/500 (1.04 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the rat skeletal muscle.The section was incubated with ab308619 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse cardiac muscle tissue staining Connexin 43 / GJA1 with ab235585 at 0.507 μg/ml, ab308619 anti-MYBPC3, and ab259345 anti-Laminin subunit alpha-2.

Panel A : anti-Connexin 43 / GJA1 (green; Opal™520), anti-MYBPC3 (magenta; Opal™570), anti-Laminin subunit alpha-2 (gray; Opal™690) on mouse cardiac muscle.

Panel B : anti-Connexin 43 / GJA1 staining intercalated discs in mouse cardiac muscle.

Panel C : anti-MYBPC3 staining cytoplasm of heart myocytes in mouse cardiac muscle.

Panel D : anti-Laminin subunit alpha-2 staining basal laminae in mouse cardiac muscle.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab235585, ab308619 and ab259345 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse cardiac muscle tissue staining Connexin 43 / GJA1 with ab235585 at 0.507 μg/ml, ab308619 anti-MYBPC3, and ab218198 anti-Dystrophin.

Panel A : anti-Connexin 43 / GJA1 (green; Opal™520), anti-MYBPC3 (magenta; Opal™570), anti-Dystrophin (gray; Opal™690) on mouse cardiac muscle.

Panel B : anti-Connexin 43 / GJA1 staining intercalated discs in mouse cardiac muscle.

Panel C : anti-MYBPC3 staining cytoplasm of heart myocytes in mouse cardiac muscle.

Panel D : anti-Dystrophin staining membrane of heart myocytes in mouse cardiac muscle.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab235585, ab308619 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The identities of the lower MW bands between 150 kDa and 37 kDa are expected to be MYBPC3 degradation products. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : 1 second

All lanes:

Western blot - Anti-MYBPC3 antibody [EPR25673-58] (ab308619) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 140 kDa

Observed band size: 150 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-MYBPC3 antibody [EPR25673-58] (AB308619)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : skeletal muscle (PMID : 23936073). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-MYBPC3 antibody [EPR25673-58] (ab308619) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat heart tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 140 kDa

Observed band size: 150 kDa

false

Exposure time: 37s