Anti-Myc tag antibody [Hyper-myc]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody [Hyper-myc] (AB289981)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) whole cell lysate labelingMyc tag with ab289981 at 1/8000 dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor^® 488) secondary antibody at 1/500 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with CRISPR-Cas9 (S. Aureas) expression vector containing a myc tag. The nuclear counter stain is DAPI (blue).

Anti-CRISPR-Cas9 rabbit monoclonal antibody (ab203933) at 1/500 dilution was used as counterstain followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150088) (red).

The negative controls are as follows :
-ve control 1 : ab289981 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150088) secondary antibody at 1/200 dilution.
-ve control 2 : ab203933 at 1/500 dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor^® 488) at 1/500 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Myc tag antibody [Hyper-myc] (AB289981)

Myc tag was immunoprecipitated from HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate with ab289981 at 1/30 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 (Input) : HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag^® whole cell lysate 2 μg

Lane 2 (+) : HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag^® whole cell lysate

Lane 3 (-) : Human IgG instead of ab289981 in HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag^® whole cell lysate.

Lane 1:

Immunoprecipitation - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (<a href='/ko/products/primary-antibodies/myc-tag-antibody-hyper-myc-mouse-igg2a-chimeric-ab289980'>ab289980</a>)

Lane 1:

Immunoprecipitation - Anti-Myc tag antibody [Hyper-myc] (ab289981)

Predicted band size: 49 kDa

Observed band size: 70 kDa

false

Exposure time: 6s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Myc tag antibody [Hyper-myc] (AB289981)

Indirect ELISA antibody dose-response curve using ab289981 with antigen at 1000 ng/ml.

• WB

Supplier Data

Western blot - Anti-Myc tag antibody [Hyper-myc] (AB289981)

Blocking and diluting buffer and concnetration are 5% NFDM/TBST.

ab289981 detects a ranged of myc tagged proteins in WB.

All lanes:

Western blot - Anti-Myc tag antibody [Hyper-myc] (ab289981) at 1/5000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 2:

HEK-293T transfected with cas9 ( S. Aureas) expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 3:

HEK-293T transfected with human SNCA140 expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 4:

HEK-293T transfected with human IDS expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 5:

HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 6:

HEK-293T transfected with human Alpha-synuclein expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

Predicted band size: 49 kDa

false

Exposure time: 1s