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AB267425

Anti-Myeloperoxidase antibody [EPR22162-183] - BSA and Azide free (Capture)

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Myeloperoxidase antibody. Carrier free. Suitable for sELISA and reacts with Human samples. Cited in 2 publications.
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Sandwich ELISA - Anti-Myeloperoxidase antibody [EPR22162-183] - BSA and Azide free (Capture) (AB267425)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Myeloperoxidase antibody [EPR22162-183] - BSA and Azide free (Capture) (AB267425)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272101).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22162-183

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB105136

Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric)

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (AB105136)

  • 4
  • 3
Sample Prep & Detection Kits

AB188285

FITC Conjugation Kit (Fast) - Lightning-Link®

Fluorescence Microscopy - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

  • 5
  • 1
Sample Prep & Detection Kits

AB201808

Gold Conjugation Kit (10 nm, 20 OD)

Conjugation - Gold Conjugation Kit (10 nm, 20 OD) (AB201808)

  • 0
  • 0
Primary Antibodies

AB272279

Anti-Neutrophil Elastase antibody [EPR22896-31] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Neutrophil Elastase antibody [EPR22896-31] - BSA and Azide free (Capture) (AB272279)

  • 0
  • 0
Primary Antibodies

AB303639

Anti-CFHR2 antibody [EPR25758-10] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-CFHR2 antibody [EPR25758-10] - BSA and Azide free (Capture) (AB303639)

  • 0
  • 0
Primary Antibodies

AB259692

Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector) (AB259692)

  • 0
  • 0
Primary Antibodies

AB5103

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (AB5103)

  • 4
  • 28
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab267425 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Myeloperoxidase. The recommended pair for sandwich ELISA is:
Capture: ab267425, Human Myeloperoxidase (MPO) Capture Antibody (unconjugated)
Detector: ab267426, Human Myeloperoxidase (MPO) Detector Antibody (unconjugated)
The reference range value is 93.75 - 6000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).
See full target information MPO

대체 명칭 보기

Myeloperoxidase, MPO
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:27263 PubMed40715596

2025

Comparative and pharmacological investigation of bEVs from eight Lactobacillales strains.

Applications

Unspecified application

Species

Unspecified reactive species

Seoah Park,Jongsoo Mok,Hye-Min Yu,Hye-Jin An,Ga-Hyun Choi,Yeon-Seon Lee,Ki-Jin Kwon,Sung-Jun Choi,Kyung-Hee Kim,Soo-Jin Kim,Joonghoon Park

Frontiers in immunology 13:815412 PubMed35242132

2022

NET Release of Long-Term Surviving Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Philipp Kolman,Laia Pagerols Raluy,Ingo Müller,Viacheslav O Nikolaev,Magdalena Trochimiuk,Birgit Appl,Hannah Wadehn,Charlotte Maria Dücker,Fabian David Stoll,Michael Boettcher,Konrad Reinshagen,Julian Trah
제품이 사용된 논문 모두 보기
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Product promise

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