AB240019

Anti-Myf5 antibody [EPR4899] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MYF5 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

BHLHC2, MYF5, Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, bHLHc2

1 이미지

Lab

Immunoprecipitation - Anti-Myf5 antibody [EPR4899] - BSA and Azide free (AB240019)

ab125078 (Purified) at 1 : 1000 dilution (0.666 μg/ml) immunoprecipitating Myf5 in Human skeletal muscle lysate.
Lane 1 (input) : Human skeletal muscle
Lane 2 (+) : 125078 & Human skeletal muscle lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab125078 in human skeletal muscle lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125078).

All lanes:

Immunoprecipitation - Anti-Myf5 antibody [EPR4899] (<a href='/ko/products/primary-antibodies/myf5-antibody-epr4899-ab125078'>ab125078</a>)

Predicted band size: 28 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4899

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab240019 is the carrier-free version of ab125078.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Myf5 also known as Myogenic Factor 5 is a member of the myogenic regulatory factor (MRF) family. It plays a critical role in muscle differentiation. Myf5 has a molecular mass of approximately 29 kDa. It is expressed predominantly in skeletal muscle tissues. The protein is observed early in muscle development marking the beginning stages where muscle precursor cells commit to becoming muscle cells.

Biological function summary

Myf5 functions as a transcription factor that initiates muscle differentiation. It acts as part of a larger complex interacting with other MRFs such as MyoD and MYF6. These factors together regulate the expression of genes necessary for myoblasts to develop into mature muscle fibers. Myf5 directly binds to the promoter regions of target genes initiating transcription that supports muscle cell lineage progression.

Pathways

The myogenesis pathway is heavily influenced by Myf5 activity. It works in tandem with MyoD in the early specification of myoblasts and their proliferation. Secondary to this Myf5 forms a feedback loop with proteins like MEF2 and Pax7 which reinforces muscle identity. Myf5 expression helps maintain the balance between myoblast proliferation and differentiation ensuring effective muscle regeneration and repair.

Muscular dystrophy exemplifies a condition closely associated with Myf5. The protein's expression levels and functionality often correlate with the disease affecting muscle development and integrity. Satellite cells which express Myf5 become important in muscle repair and dysfunctional Myf5 can contribute to disease progression. Another disorder influenced by Myf5 is rhabdomyosarcoma where aberrant expression disrupts normal myogenic regulatory pathways alongside MyoD dysregulation potentially leading to tumorigenesis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed : 29887215). Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

See full target information MYF5

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Autophagy 19:2575-2577 PubMed37194363

2023

Knockout validation of LAMP2A antibodies for immunostaining in human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Zhou,Vera Shirokova,Vitaliy O Kaminskyy,Eva Berenger,Elena Kochetkova,Erik Norberg,Maria Genander,Helin Vakifahmetoglu-Norberg

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myf5 antibody [EPR4899] - BSA and Azide free

Immunoprecipitation - Anti-Myf5 antibody [EPR4899] - BSA and Azide free (AB240019)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise