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AB243451

Anti-MYO9B antibody

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Rabbit Polyclonal MYO9B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MYO9B aa 1350 to C-terminus.

대체 명칭 보기

MYR5, MYO9B, Unconventional myosin-IXb, Unconventional myosin-9b

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO9B antibody (AB243451)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO9B antibody (AB243451)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for MYO9B with ab243451 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MYO9B antibody (AB243451)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MYO9B antibody (AB243451)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling MYO9B using ab243451 at 4 μg/ml (green) in ICC/IF. Nuclear probe is visualized in blue.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MYO9B aa 1350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13459

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보완 제품

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Primary Antibodies

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Primary Antibodies

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein MYO9B also known as myosin IXb is part of the unconventional myosin family and has a molecular mass of approximately 290 kDa. It is an actin-based motor protein that operates in the cellular cytoskeleton to facilitate various cellular processes. MYO9B is expressed in tissues such as the kidney liver and particularly high in the intestinal epithelium where it plays critical roles in maintaining cell shape and motility.
Biological function summary

MYO9B modulates the dynamics of the actin cytoskeleton influencing cell movement and polarity. This myosin acts as a molecular motor converting chemical energy into mechanical work driving intracellular transport and signaling processes. It also contains a Rho GTPase-activating protein (RhoGAP) domain implicating it in the regulation of Rho family GTPases which are pivotal for actin organization. MYO9B does not function alone but interacts with other proteins within molecular complexes to maintain cellular integrity.

Pathways

MYO9B participates in signaling cascades that control cytoskeleton rearrangement and cellular adhesion. Notably MYO9B plays roles in the RhoA signaling pathway essential for actin-myosin organization and cellular contraction. It accomplishes this by acting in opposition to proteins like ROCK1 impacting cellular tension and morphology.

Alterations in MYO9B have been linked to inflammatory bowel disease (IBD) and celiac disease. MYO9B interacts with other proteins involved in maintaining intestinal barrier function notably TGF-beta signaling components that play a role in gut immune responses. Dysregulation in this protein affects epithelial cell function contributing to the pathogenesis of these gastrointestinal disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions (PubMed : 9490638). Also acts as a GTPase activator for RHOA (PubMed : 26529257, PubMed : 9490638). Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed : 26529257).
See full target information MYO9B
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 28:e18215 PubMed38509755

2024

CCDC157 is essential for sperm differentiation and shows oligoasthenoteratozoospermia-related mutations in men.

Applications

Unspecified application

Species

Unspecified reactive species

Huimei Zheng,Chenjia Gong,Jingping Li,Jiaru Hou,Xinhan Gong,Xinhai Zhu,Huan Deng,Haoyue Wu,Fengbin Zhang,Qinghua Shi,Jianteng Zhou,Baolu Shi,Xiaohang Yang,Yongmei Xi
제품이 사용된 논문 모두 보기
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Product promise

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