AB212662

Anti-MyoD1 antibody [5.8A] - BSA free

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(1 리뷰)

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(3 제품이 사용된 논문 )

Mouse Monoclonal MYOD1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Myod1.

대체 명칭 보기

BHLHC1, MYF3, MYOD, MYOD1, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3, bHLHc1, Myf-3

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.8A] - BSA free (AB212662)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.8A] - BSA free (AB212662)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Rhabdomyosarcoma tissue section labeling MyoD1 with ab212662 at 1 μg/mL.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

5.8A

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

Immunogen

Recombinant Full Length Protein corresponding to Mouse Myod1.

Epitope

The epitope of ab212662 maps between amino acid 180-189 in the C terminal of mouse MyoD1 protein.

Specificity

ab212662 does not cross react with myogenin, Myf5, or Myf6.

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대체품

Primary Antibodies

AB209976

Anti-MyoD1 antibody

Lab Essentials

IHC - Wholemount - Anti-MyoD1 antibody (AB209976)

0
0

Primary Antibodies

AB16148

Anti-MyoD1 antibody [5.2F]

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.2F] (AB16148)

3
18

Primary Antibodies

AB198251

Anti-MyoD1 antibody - C-terminal

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody - C-terminal (AB198251)

0
0

Primary Antibodies

AB64159

Anti-MyoD1 antibody

Lab Essentials

Western blot - Anti-MyoD1 antibody (AB64159)

3
3

Primary Antibodies

AB133627

Anti-MyoD1 antibody [EPR6653-131]

RabMAb|Recombinant|Lab Essentials|Advanced Validation|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (AB133627)

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11

Primary Antibodies

AB240073

Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free

Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)

0
0

Primary Antibodies

AB203383

Anti-MyoD1 antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody (AB203383)

3
3

보완 제품

Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

4
56

Primary Antibodies

AB124800

Anti-Myogenin antibody [EPR4789]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (AB124800)

5
3

Primary Antibodies

AB219650

Anti-PRAME antibody [EPR20330]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAME antibody [EPR20330] (AB219650)

0
0

Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

5
20

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>for 30 minutes at room temperature.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from bioreactor concentrate.

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MyoD1 also known as Myogenic Differentiation 1 is a master regulator of muscle differentiation. It functions mechanically as a transcription factor binding to DNA at specific sequences thereby activating the transcription of genes necessary for muscle tissue development. MyoD1 has a molecular weight of approximately 45 kDa. It is expressed in skeletal muscle tissues and also detected in some non-muscle tissues though at lower levels. MyoD1 is essential in the early stages of muscle cell lineage commitment.

Biological function summary

MyoD1 plays an important role in muscle differentiation by activating muscle-specific genes. It belongs to the myogenic regulatory factor family and often operates within a protein complex alongside Myf5 and myogenin aiding in the conversion of mesodermal stem cells into muscle cells. These partnerships enhance its ability to initiate the muscle-specific gene expression that drives myogenesis. MyoD1 acts like a molecular switch shifting cells into the pathway leading to muscle formation.

Pathways

MyoD1 is an integral component of myogenesis and muscle regeneration pathways. MyoD1 interacts with key pathway components such as Myf5 and MRF4 which reinforce MyoD1 activity in muscle tissue lineage determination and cell cycle arrest during differentiation. The activity of MyoD1 in these pathways highlights its role in skeletal muscle growth. MyoD1 also demonstrates functional redundancy with Myogenin as both share overlapping roles in these critical pathways.

MyoD1 is associated with muscular dystrophy and some sarcomas. Aberrant expression or mutations in MyoD1 can disrupt normal muscle differentiation contributing to muscle-related disorders like rhabdomyosarcoma a form of cancer comprising cells resembling skeletal muscle. Its relationship with other proteins like Myf5 further highlights its role in such pathological conditions as faults in these connections can amplify or alter disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).

See full target information MYOD1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Advanced biology 7:e2200322 PubMed36895072

2023

Differentiation of Peritubular Myoid-Like Cells from Human Induced Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan Robinson,Anne Haegert,Yen-Yi Li,Tunc Morova,Angelina Yuan Yuan Zhang,Luke Witherspoon,Faraz Hach,Stephanie M Willerth,Ryan Flannigan

BMC biology 21:19 PubMed36726129

2023

Integrative single-cell RNA-seq and ATAC-seq analysis of myogenic differentiation in pig.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Cai,Bin Hu,Xiaoyu Wang,Tongni Liu,Zhuhu Lin,Xian Tong,Rong Xu,Meilin Chen,Tianqi Duo,Qi Zhu,Ziyun Liang,Enru Li,Yaosheng Chen,Jianhao Li,Xiaohong Liu,Delin Mo

Journal of cellular physiology 235:5649-5665 PubMed31960436

2020

Resistance training-induced muscle hypertrophy is mediated by TGF-β1-Smad signaling pathway in male Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rohollah Nikooie,Sohil Jafari-Sardoie,Vahid Sheibani,Amir Nejadvaziri Chatroudi

제품이 사용된 논문 모두 보기

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Product promise

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