Anti-MyoD1 antibody [EPR29627-738]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (AB323377)

Immunohistochemical analysis of paraffin-embedded Mouse E12 embryo tissue labeling MyoD1 with ab323377 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on skeletal muscle of mouse E12 embryo. The section was incubated with ab323377 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (AB323377)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MyoD1 with ab323377 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse spleen. The section was incubated with ab323377 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR29627-738] (AB323377)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MyoD1 with ab323377 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cardiac muscle. The section was incubated with ab323377 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-MyoD1 antibody [EPR29627-738] (AB323377)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa (PMID : 17028574 )

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377) at 1/1000 dilution

Lane 1:

Mouse E14 forelimb tissue lysate at 40 µg

Lane 2:

Mouse E14 hindlimb tissue lysate at 40 µg

Lane 3:

Mouse embryo tissue lysate at 40 µg

Lane 4:

RD(human muscle mtinucleated spindle-shaped cell) whole lysate at 40 µg

Lane 5:

HeLa (human cervical adenocarcinoma epithelial cell) whole lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 47 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MyoD1 antibody [EPR29627-738] (AB323377)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of MyoD1 is downregulated in response to C2C12 differentiation for 5 days treatment(DOI : 10.4236/cellbio.2013.23016)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377) at 1/1000 dilution

Lane 1:

Untreated C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 2:

C2C12 differentiation to muscle for 5 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 47 kDa,36 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-MyoD1 antibody [EPR29627-738] (AB323377)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : brain, heart, kidney, spleen(HPA data)

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377) at 1/1000 dilution

Lane 1:

Mouse E12 embryo tissue lysate at 20 µg

Lane 2:

Mouse E14 embryo tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Lane 6:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 47 kDa,36 kDa

true

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-MyoD1 antibody [EPR29627-738] (AB323377)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse MYF5, MYF6 or MYOG.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-MyoD1 antibody [EPR29627-738] (ab323377) at 1/1000 dilution

Lane 1:

His-tagged mouse MYOD1 recombinant protein at 10 ng

Lane 2:

His-tagged mouse MYF5 recombinant protein at 10 ng

Lane 3:

His-tagged mouse MYF6 recombinant protein at 10 ng

Lane 4:

His-tagged mouse MYOG recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa,40 kDa

false

Exposure time: 37s