AB150386

Anti-Myosin VIIa/MYO7A antibody [EPR7497]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(12 제품이 사용된 논문 )

Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) is a rabbit monoclonal antibody detecting Myosin VIIa/MYO7A in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Immunofluorescence analysis of A97 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Intracellular flow cytometric analysis of permeabilized Y79 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.

Unknown

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

All lanes:

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) at 1/1000 dilution

Lane 1:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Lane 2:

Rat testis tissue lysate at 10 µg

Lane 3:

Mouse testis tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat-anti rabbit antibody at 1/2000 dilution

Predicted band size: 254 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7497

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Myosin VIIa/MYO7A?
Anti-Myosin VIIa/MYO7A [EPR7497] (ab150386) specifically detects a band for Myosin VIIa/MYO7A (UniProt: Q13402) at a molecular weight of 254kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR7497] also available for your convenience: ab150386, Carrier free - ab248957, APC - ab319262, PE - ab319410, Alexa Fluor® 488 - ab319544, Alexa Fluor® 647 - ab319657, Alexa Fluor® 594 - ab319761, Alexa Fluor® 555 - ab319903

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Myosin VIIa also known as MYO7A or myosin 7a is a motor protein that plays an important role in cellular transport and movement. It belongs to the myosin superfamily and has a molecular mass of approximately 250 kDa. This protein is widely expressed in sensory cells especially in the inner ear and the retina. In reproductive tissues it aids in sperm tail movements. The mechanical function of MYO7A involves ATP-dependent interactions with actin filaments leading to motor activity and cargo transport within cells.

Biological function summary

Myosin VIIa is essential for the maintenance of stereocilia structure and function in the sensory hair cells of the cochlea. It forms part of a multiprotein complex that stabilizes the actin filaments contributing to auditory signal transduction. Furthermore it is critical in the retinal pigment epithelium for normal visual processes. The protein ensures the correct intracellular localization of key components affecting cell signaling and environmental responsiveness.

Pathways

Myosin VIIa is involved in both auditory and visual signal pathways key for sensory perception. In the auditory pathway it interacts with harmonin and cadherin 23 which also play a part in mechanotransduction. In the visual pathway it affects signaling processes that rely on precise positioning of visual pigments. These interactions highlight its role in maintaining proper sensory functions in complex signaling pathways.

Mutations in MYO7A can lead to conditions such as Usher syndrome type 1B and nonsyndromic deafness. Usher syndrome is characterized by combined deafness and vision loss due to retinal degeneration. Non-syndromic hearing loss involves hearing impairment without other clinical disabilities. Both disorders demonstrate the critical importance of myosin VIIa in auditory and visual neurons. Connections between MYO7A and cadherin proteins further implicate these pathways in disease pathology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity (By similarity). Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.

See full target information MYO7A

대체 명칭 보기

USH1B, MYO7A, Unconventional myosin-VIIa

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제품이 사용된 논문 (12)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40563254

2025

Ferulic Acid Combines with Ascorbic Acid to Target MMP9 to Attenuate Cisplatin-Induced Ototoxicity Through the p38MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guojun Yang,Na Hu,Jie Gao,Xinzhi Li,Bin Zhang,Ketao Ma

PloS one 20:e0320634 PubMed40198625

2025

Ptgds downregulation protect vestibular hair cells from aminoglycoside-induced vestibulotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Chen,Zhimin Zhao,Jinghong Han,Yue Zhang,Guohui Nie

Cell communication and signaling : CCS 23:121 PubMed40045370

2025

Impact of POU3F4 mutation on cochlear development and auditory function.

Applications

Unspecified application

Species

Unspecified reactive species

Jiong Dang,Panpan Bian,Chao Chen,Chi Chen,Wenqi Shan,Luhang Cai,Yong Li,Huan Tan,Baicheng Xu,Minxin Guan,Yufen Guo

Cell biology and toxicology 41:31 PubMed39820591

2025

Innovative treatment of age-related hearing loss using MSCs and EVs with Apelin.

Applications

Unspecified application

Species

Unspecified reactive species

Shengqun Xu,Dongliang Liu,Fang Zhang,Yuan Tian

International journal of molecular sciences 25: PubMed39201392

2024

TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Assim Hayderi,Mulugeta Melkie Zegeye,Sare Meydan,Allan Sirsjö,Ashok Kumar Kumawat,Liza U Ljungberg

Cell death & disease 14:605 PubMed37704645

2023

Intranasal delivery of NGF rescues hearing impairment in aged SAMP8 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Castelli,Michele d'Angelo,Francesca Zazzeroni,Davide Vecchiotti,Edoardo Alesse,Daria Capece,Laura Brandolini,Franca Cattani,Andrea Aramini,Marcello Allegretti,Annamaria Cimini

BMC pharmacology & toxicology 24:16 PubMed36882858

2023

miR-34a/DRP-1-mediated mitophagy participated in cisplatin-induced ototoxicity via increasing oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Wang,Hanqing Lin,Weibiao Kang,Lingfei Huang,Sisi Gong,Tao Zhang,Xiaotong Huang,Feinan He,Yongyi Ye,Yiyang Tang,Haiying Jia,Haidi Yang

Frontiers in molecular neuroscience 15:963083 PubMed35992197

2022

Pitavastatin protects against neomycin-induced ototoxicity through inhibition of endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhao Wu,Wei Meng,Ming Guan,Xiaolong Zhao,Chen Zhang,Qiaojun Fang,Yuhua Zhang,Zihui Sun,Mingjing Cai,Dongdong Huang,Xuechun Yang,Yafeng Yu,Yong Cui,Shuangba He,Renjie Chai

Bioengineered 13:6729-6739 PubMed35246011

2022

Small activating RNA activation of ATOH1 promotes regeneration of human inner ear hair cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Li Zhang,Moorim Kang,Jian-Cheng Wu,Meng-Yao Xie,Ruo-Yan Xue,Qi Tang,Hua Yang,Long-Cheng Li

International journal of molecular sciences 23: PubMed35163494

2022

Usher Syndrome Belongs to the Genetic Diseases Associated with Radiosensitivity: Influence of the ATM Protein Kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Joëlle Al-Choboq,Mélanie L Ferlazzo,Laurène Sonzogni,Adeline Granzotto,Laura El-Nachef,Mira Maalouf,Elise Berthel,Nicolas Foray

제품이 사용된 논문 모두 보기

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myosin VIIa/MYO7A antibody [EPR7497]

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Flow Cytometry (Intracellular) - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

관련 conjugated 항체와 다양한 조성의 항체 (7)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (12)

Product promise