Anti-N-terminal LPHN2 antibody [EPR29557-9]

• WB

Supplier Data

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (AB324484)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : U-937, HEL, K-562

The samples are unboiled.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 15483624).

The observed band is the N-terminal LPHN2.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (ab324484) at 1/1000 dilution

Lane 1:

SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HuTu 80 (human Small intestine; Duodenum epithelial) whole cell lysate at 20 µg

Lane 5:

HEL (human erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 6:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 7:

NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 120 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (AB324484)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle (PMID : 10030676)

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The samples are unboiled.

The observed band is the N-terminal LPHN2.

All lanes:

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (ab324484) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 120 kDa,124 kDa

true

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (AB324484)

Dot blot analysis of N-terminal LPHN2 using ab324484 at 1 : 1000 (0.495 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human LPHN2 recombinant fragment
Lane2 : His-tagged human LPHN1 recombinant fragment
Lane3 : His-tagged human LPHN3 recombinant fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human LPHN1 or LPHN3.

#In Dot Blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (ab324484) at 1/1000 dilution

Lane 1:

His-tagged human LPHN2 recombinant fragment

Lane 2:

His-tagged human LPHN1 recombinant fragment

Lane 3:

His-tagged human LPHN3 recombinant fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (AB324484)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : EL4

The samples are unboiled.

The observed band is the N-terminal LPHN2.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1-2 : 125 seconds, lane3 : 48 seconds

All lanes:

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (ab324484) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 40 µg

Lane 3:

C6 (rat glial tumor glial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 120 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (AB324484)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle(PMID : 10030676)

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The samples are unboiled.

The observed band is the N-terminal LPHN2.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (ab324484) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 120 kDa,36 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (AB324484)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The samples are unboiled.

This antibody does not cross-react with human LPHN1 or LPHN3.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (ab324484) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human LPHN2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human LPHN1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human LPHN3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 120 kDa,150 kDa,36 kDa,60 kDa

false

Exposure time: 3s