AB151230

Anti-N6-methyladenosine (m6A) antibody

Name: Anti-N6-methyladenosine (m6A) antibody
Brand: Abcam Limited
SKU: AB151230
Rating: 5 (3 reviews)

(3 리뷰들)

(217 제품이 사용된 논문 )

Anti-N6-methyladenosine (m6A) antibody (ab151230) is a rabbit polyclonal antibody detecting N6-methyladenosine (m6A).

- Over 110 publications

대체 명칭 보기

N6-methyladenosine, m6A

2 이미지

Nucleotide Array

Lab

Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (AB151230)

All batches of ab151230 are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.

2Ome-m6A - N6-methyladenosine

2Ome-m6A_unmod - unmodified adenosine

Lab

Western blot - Anti-N6-methyladenosine (m6A) antibody (AB151230)

Western blot performed under reducing conditions.

All lanes:

Western blot - Anti-N6-methyladenosine (m6A) antibody (ab151230) at 1 µg/mL

Lane 1:

N6-Methyladenosine conjugated BSA at 0.1 µg

Lane 2:

Adenosine conjugated BSA at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

true

Exposure time: 1min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Applications

Nucleotide Array, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "NucleotideArray" : {"fullname" : "Nucleotide Array", "shortname":"Nucleotide Array"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "NucleotideArray-species-checked": "guaranteed", "NucleotideArray-species-dilution-info": "2 µg/mL", "NucleotideArray-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.

The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

Product Specifications
Anti-N6-methyladenosine (m6A) antibody (ab151230) is a rabbit polyclonal antibody and is validated for use in Nucleotide Array.
Anti-N6-methyladenosine (m6A) antibody (ab151230) specifically detects N6-methyladenosine (m6A) (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-N6-methyladenosine (m6A) antibody (ab151230) has high sensitivity and specificity.
Anti-N6-methyladenosine (m6A) antibody (ab151230) has been cited over 111 times in peer reviewed journals and is trusted by the scientific community.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).

Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information N6-methyladenosine (m6A)

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제품이 사용된 논문 (217)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomolecules 15: PubMed40867632

2025

Apelin-13-Mediated Upregulation of METTL3 Ameliorates Alzheimer's Disease via Inhibiting Neuroinflammation Through m6A-Dependent Regulation of lncRNA BDNF-AS.

Applications

Unspecified application

Species

Unspecified reactive species

Li Han,Siwen Wei,Rong Wang,Yiran Liu,Yi Zhong,Juan Fu,Huaiqing Luo,Meihua Bao

Frontiers in pharmacology 16:1631076 PubMed40771928

2025

Ginsenoside Rb1 mitigates atherosclerosis in part through modulating FTO-mediated mA RNA modification in NETs-induced endothelial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenni Yang,Minqi Xiong,Xinmiao Tang,Peiwei Wang,Jingang Cui,Yu Chen,Teng Zhang

Viruses 17: PubMed40573422

2025

Strain-Divergent m6A Landscapes Modulate Nipah Virus Replication and METTL3 Inhibition Attenuates Virulence.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Luo,Zhen Chen,Fang Zhang,Haibin Liu,Fang Huang,Xueyan Zhang,Jiangpeng Feng,Shuang Ding,Lishi Liu,Wuxiang Guan,Aiping Zeng,Haojie Hao

Nature communications 16:4930 PubMed40436842

2025

An RNA transmethylation pathway governs kidney nephrogenic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Harini Ramalingam,Jesus Alvarez,Andrea Flaten,Patricia Cobo-Stark,Nicholas Foster,Elyse Grilli,Ronak Lakhia,Karam Aboudehen,Thomas Carroll,Vishal Patel

American journal of reproductive immunology (New York, N.Y. : 1989) 93:e70102 PubMed40435048

2025

Knockdown of TRIM47 Overcomes Paclitaxel Resistance in Ovarian Cancer by Suppressing the TGF-β Pathway via PPM1A.

Applications

Unspecified application

Species

Unspecified reactive species

Hezhu Wang,Shengjun Chen,Feifei Xu,Xiaojing Chen

Science advances 11:eadq3052 PubMed40435251

2025

FTO regulates ELK3-mediated metabolic rewiring and represents a unique therapeutic target in T cell leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Huang,Xinlu Li,Jinlian Luo,Chuan Gao,Mengjie Yang,Jin Xu,Ting Xie,Zhi Chen,Donghai Wang,Yuan Wang,Hua-Bing Li,Jinyan Huang,Yu Liu,Haojian Zhang,Panagiotis Ntziachristos,Yun Zhao,Guoliang Qing,Hudan Liu

Translational lung cancer research 14:1351-1370 PubMed40386722

2025

was stabilized through N-methyladenosine methylation and exerted its anticancer effects in lung squamous cell carcinoma by inhibiting SIRT1 activity via CCAR2.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Yan,Wingshing Wong,Jinsong Lei,Dachuan Liang,Jie Yang,Li Gong,Rossana Berardi,Shuqin Dai,Junye Wang

Molecular therapy. Nucleic acids 36:102533 PubMed40291376

2025

An inflammation-associated lncRNA induces neuronal damage via mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ane Olazagoitia-Garmendia,Henar Rojas-Márquez,Tim Trobisch,Cristina Moreno-Castro,Ariadne Rodriguez Etxebarria,Jon Mentxaka,Ajai Tripathi,Bibo Yang,Itziar Martin Ruiz,Juan Anguita,J Javier Meana,Yiliang Ding,Ranjan Dutta,Lucas Schirmer,Mariana Igoillo-Esteve,Izortze Santin,Ainara Castellanos-Rubio

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 36:269-279 PubMed40241612

2025

Regulatory Role of IGF2BP2 in Intestinal Mucosal Barrier Dysfunction in Ulcerative Colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruifan Li,Bin Gu,Anli Lv

Nature communications 16:2925 PubMed40133293

2025

FTO suppresses DNA repair by inhibiting PARP1.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Zhu,Jing Zhi Anson Tan,Lingrui Zhang,He Huang,Sooraj S Das,Flora Cheng,Pranesh Padmanabhan,Mathew J K Jones,Mihwa Lee,Albert Lee,Jocelyn Widagdo,Victor Anggono

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N6-methyladenosine (m6A) antibody

Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (AB151230)

Western blot - Anti-N6-methyladenosine (m6A) antibody (AB151230)

주요 정보

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (217)

Product promise