Anti-NAA38 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAA38 antibody (AB236965)

Paraffin-embedded human kidney tissue stained for NAA38 using ab236965 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NAA38 antibody (AB236965)

PC-3 (human prostate adenocarcinoma cell line) cells stained for NAA38 (Green) using ab236965 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

• WB

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Western blot - Anti-NAA38 antibody (AB236965)

All lanes:

Western blot - Anti-NAA38 antibody (ab236965) at 1/500 dilution

All lanes:

Mouse kidney tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 14 kDa

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