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AB234978

Anti-NAGPA antibody

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Rabbit Polyclonal NAGPA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAGPA aa 300 to C-terminus.

대체 명칭 보기

N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase, Mannose 6-phosphate-uncovering enzyme, Phosphodiester alpha-GlcNAcase, NAGPA

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGPA antibody (AB234978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGPA antibody (AB234978)

Paraffin-embedded human colon cancer tissue stained for NAGPA with ab234978 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NAGPA aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UK23

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보완 제품

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB239364

Anti-Cdk9 antibody [EPR22956-37] - ChIP Grade

20ul selling size|BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody [EPR22956-37] - ChIP Grade (AB239364)

  • 0
  • 0
Primary Antibodies

AB50148

Anti-beta Glucuronidase (GUS) antibody - Aminoterminal end

  • 0
  • 0
Primary Antibodies

AB221353

Anti-UNCX antibody

Immunocytochemistry/ Immunofluorescence - Anti-UNCX antibody (AB221353)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NAGPA also known as N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase plays an important role in the modification of lysosomal proteins. This enzyme's molecular mass is around 62 kDa and it primarily operates within the Golgi apparatus. It is widely expressed across various tissues making it an essential component in the cell's processing and trafficking functions. Other terminology includes hexosaminidase a which reflects its function in cleaving glucosamine residues.
Biological function summary

NAGPA participates in the formation and processing of mannose-6-phosphate recognition markers essential for lysosomal protein targeting. It functions independently rather than as part of a multi-protein complex. The enzyme helps remove a N-acetylglucosamine (GlcNAc) group exposing the mannose-6-phosphate which is a critical step required for lysosomal enzymes to be recognized and transported to the lysosome.

Pathways

Which include NAGPA the enzyme plays a significant role in the lysosomal enzyme targeting pathway and glycoprotein metabolic processes. The mannose-6-phosphate pathway is important for directing enzymes to lysosomes requiring precise interaction between NAGPA and the phosphotransferase proteins responsible for initial phosphorylation. Its regulatory actions ensure lysosomal enzymes are properly targeted and activated.

Malfunction or deficiencies in NAGPA can lead to issues such as mucolipidosis III a disorder characterized by improper lysosomal enzyme targeting. NAGPA's connection with hexosaminidase b involved in the breakdown of GM2 gangliosides also links it to disorders such as Sandhoff disease. This highlights NAGPA's pivotal role in maintaining lysosomal function and contributing to overall cellular health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.
See full target information NAGPA
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2416590 PubMed40298908

2025

Nuclear-Localized BCKDK Facilitates Homologous Recombination Repair to Support Breast Cancer Progression and Therapy Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Haiying Liu,Jiaqian Feng,Tingting Pan,Pinggen Zhang,Ling Ye,Zetan Jiang,Zilong Zhou,Qiankun Mao,Jian Li,Xinyi Yang,Ping Gao,De Huang,Huafeng Zhang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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