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AB185082

Anti-Naked1 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal Naked1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NKD1 aa 150-300.

대체 명칭 보기

NKD, PP7246, NKD1, Protein naked cuticle homolog 1, Naked-1, hNkd, hNkd1

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Naked1 antibody (AB185082)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Naked1 antibody (AB185082)

Immunohistochemistry analysis human smooth muscle tissue labeling Naked1 at the cytoplasm and nucleus of smooth muscle cells with ab185082 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Naked1 antibody (AB185082)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Naked1 antibody (AB185082)

Immunocytochemistry analysis HepG2 (human liver hepatocellular carcinoma cell line) whole cells labeling Naked1 at the nucleoli fibrillar center and cytosol with ab185082 at 2 μg/ml.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NKD1 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q969G9

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대체품

Primary Antibodies

AB237535

Anti-Naked1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Naked1 antibody (AB237535)

  • 0
  • 0

보완 제품

Primary Antibodies

AB32197

Anti-Axin 2 antibody

Western blot - Anti-Axin 2 antibody (AB32197)

  • 4
  • 15
Primary Antibodies

AB61034

Anti-DKK1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody (AB61034)

  • 5
  • 5
Primary Antibodies

AB254588

Anti-FZD10 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FZD10 antibody (AB254588)

  • 0
  • 0
Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

  • 5
  • 75
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Naked1 also known as Nkd1 is a protein with a mass of approximately 48 kDa. It acts as an antagonist in the Wnt signaling pathway by binding to Dishevelled an intracellular protein that transmits Wnt signals. Naked1 is expressed in various tissues including the heart brain and muscles. In addition to its role in the Wnt pathway Naked1 serves as a regulatory protein providing significant control over signal transduction that influences cell fate decisions.
Biological function summary

The function of Naked1 involves modulating the activity of the Wnt/β-catenin signaling pathway. It forms part of a negative feedback loop that prevents excessive pathway activation. Naked1 dynamically interacts with components of this pathway to inhibit or fine-tune signaling transduction ensuring that cellular processes such as proliferation differentiation and migration occur correctly. This interaction is critical in developing tissues and maintaining homeostasis in adult organisms.

Pathways

Naked1 functions within the Wnt/β-catenin pathway which is significant for cell and tissue development and homeostasis. It helps regulate this pathway by interacting with Dishevelled proteins and it also closely influences the activity of Casein kinase 1 another important component of the pathway. This regulatory role ensures a balanced signaling environment which is necessary to prevent pathological conditions associated with dysregulated Wnt signaling.

Improper regulation or expression of Naked1 associates with cancer and developmental abnormalities. Naked1 interacts with and affects proteins like β-catenin which when unregulated contributes to oncogenesis. Studies suggest that abnormalities in Naked1 expression levels may lead to tumorigenesis through unchecked cellular proliferation and migration particularly in colorectal cancer. Furthermore variations in Naked1 function may contribute to congenital disorders by disrupting the tightly controlled Wnt signaling pathway during fetal development.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.
See full target information NKD1
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Genetics research 2023:1184101 PubMed36969985

2023

Clinical Significance of NKD Inhibitor of WNT Signaling Pathway 1 (NKD1) in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Li,Ruiying Gao,Weizhong Huangfu,Fang Zhang,Ruixia Wang

Cancer medicine 10:7383-7394 PubMed34547189

2021

Identification of colon tumor marker NKD1 via integrated bioinformatics analysis and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wang,Chunxia Yang,Wenjing Li,Ying Shen,Jianzhong Deng,Wenbin Lu,Jianhua Jin,Yongping Liu,Qian Liu

Osteoarthritis and cartilage 27:140-147 PubMed30223022

2018

CRISPR-Cas9 targeting of MMP13 in human chondrocytes leads to significantly reduced levels of the metalloproteinase and enhanced type II collagen accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

C I Seidl,T A Fulga,C L Murphy
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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