Anti-NANS antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NANS antibody (AB236783)

Paraffin-embedded human adrenal gland tissue stained for NANS using ab236783 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NANS antibody (AB236783)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for NANS (green) using ab236783 at 1/66 dilution in ICC/IF, followed by an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody. Counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NANS antibody (AB236783)

Paraffin-embedded human gastric cancer tissue stained for NANS using ab236783 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.