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AB234002

Anti-NAPSIN A antibody [NAPSA/1238]

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Mouse Monoclonal NAPSIN A antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NAPSA aa 150-300.

대체 명칭 보기

NAP1, NAPA, NAPSA, Napsin-A, Aspartyl protease 4, Napsin-1, TA01/TA02, ASP4, Asp 4

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [NAPSA/1238] (AB234002)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [NAPSA/1238] (AB234002)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for NAPSIN A using ab234002 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-NAPSIN A antibody [NAPSA/1238] (AB234002)
  • Protein Array

Unknown

Protein Array - Anti-NAPSIN A antibody [NAPSA/1238] (AB234002)

ab234002 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Western blot - Anti-NAPSIN A antibody [NAPSA/1238] (AB234002)
  • WB

Supplier Data

Western blot - Anti-NAPSIN A antibody [NAPSA/1238] (AB234002)

All lanes:

Western blot - Anti-NAPSIN A antibody [NAPSA/1238] (ab234002) at 2 µg/mL

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 3:

A549 (human chronic lung carcinoma cell line) cell lysate

Predicted band size: 45 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

NAPSA/1238

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, Protein Array, WB

applications

Immunogen

Recombinant Fragment Protein within Human NAPSA aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

O96009

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>(Primary incubation for 60 minutes at RT).</p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
Purified from rabbit anti-serum by Protein A.
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Napsin A also known as napsin or napsine is an aspartic proteinase with a molecular weight of approximately 38 kDa. This enzyme is primarily expressed in the lung tissue renal tubules and in some cases thymus and spleen. Napsin A plays a significant role in protein catabolism by degrading specific peptide bonds facilitating protein turnover and maintenance of cellular homeostasis. It is often useful in identifying cell types being napsin A positive in certain cells and napsin A negative in others.
Biological function summary

Napsin A functions in the maturation of pulmonary surfactant proteins which are essential for normal lung function. Napsin A cleaves surfactant protein B an important component of the surfactant system in alveolar air spaces allowing proper lung mechanics. While napsin A operates in concert with other enzymes it does not form part of a larger complex. Its role impacts cellular processes related to respiratory physiology and contributes to the regulation of protein breakdown within the cell.

Pathways

Napsin A is involved in respiratory and proteolytic pathways influencing lung physiology and systemic protein metabolism. One important pathway is the surfactant metabolism pathway which ensures adequate surfactant levels for maintaining lung function. Napsin A is related to surfactant proteins like surfactant protein B through this pathway facilitating their activation and processing.

Napsin A serves as a biomarker for certain lung adenocarcinomas where napsin A positive lung tumors often indicate differential diagnosis between primary lung cancer and metastases from other sites. Napsin A's expression is linked to the presence of adenocarcinoma a specific type of lung cancer. In renal disorders napsin A expression helps distinguish renal cell carcinoma from other renal masses where its expression pattern relates to other proteins like thyroid transcription factor-1 in diagnosing specific cancer types.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

May be involved in processing of pneumocyte surfactant precursors.
See full target information NAPSA

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Bioactive materials 50:364-381 PubMed40589547

2025

In situ implantation of type II collagen-based double-layer scaffolds for Articular Osteochondral Regeneration comprising hyaline cartilage and vascularized subchondral bones.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Ye Huang,Xu Hu,Yulei Mu,Huiqun Zhou,Liang Ma,Bangheng Liu,Hang Yao,Xieyuan Jiang,Dong-An Wang
제품이 사용된 논문 모두 보기

Product promise

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