AB241487

Anti-NAT13 antibody

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Rabbit Polyclonal NAT13 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NAA50 aa 100-200.

대체 명칭 보기

MAK3, NAT13, NAT5, NAA50, N-alpha-acetyltransferase 50, hNaa50p, N-acetyltransferase 13, N-acetyltransferase 5, N-acetyltransferase san homolog, N-epsilon-acetyltransferase 50, NatE catalytic subunit, hNAT5, hSAN

2 이미지

Supplier Data

Immunoprecipitation - Anti-NAT13 antibody (AB241487)

NAT13 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241487 at 20 μL per reaction. Western blot was perfromed on the immunoprecipitate using ab241487 at 1/250 dilution.

Lane 1 : ab241487 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NAT13 antibody (ab241487)

Predicted band size: 19 kDa

false

Supplier Data

Western blot - Anti-NAT13 antibody (AB241487)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-NAT13 antibody (ab241487) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 19 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NAA50 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9GZZ1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "20 μl/mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to NAT13 immobilized on solid support

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The N-acetyltransferase 13 (NAT13) also known as GCN5-related N-acetyltransferase-like 3 is a protein involved in acetylation processes. It has a molecular mass of about 25 kDa. NAT13 is known for its specific role in the acetylation of polyamines important for cellular metabolism. This protein is expressed in various tissues with significant presence in the liver and kidney. The widespread expression suggests its importance in maintaining normal cellular function.

Biological function summary

NAT13 exerts its effects on cellular growth modulation and proliferation. NAT13 functions as a part of the enzyme complexes that regulate the acetylation process modifying substrates in the cell. This modification impacts protein function stability and interaction with other cellular components. NAT13's enzymatic activity influences cell cycle regulation and is critical for normal cell growth and division.

Pathways

NAT13 influences polyamine biosynthesis and metabolism which are necessary for cell replication and growth. One of the significant pathways involving NAT13 is the spermidine and spermine biosynthesis pathway which interacts with the polyamine oxidase enzyme. These interactions help to balance cellular growth signals and maintain cellular homeostasis. Another associated pathway is histone acetylation impacting gene expression regulation and being correlated with the GCN5 protein.

NAT13 has connections to colorectal cancer and hepatocellular carcinoma due to its role in cell cycle regulation and proliferation. Aberrant activity of NAT13 can disrupt normal acetylation patterns leading to uncontrolled cell growth associated with tumorigenesis. Additionally NAT13's dysregulation links with proteins such as c-Myc known for their part in oncogenic processes. Understanding NAT13's activity offers insights into the molecular mechanisms behind these cancers and potential therapeutic targets.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine (PubMed : 19744929, PubMed : 21900231, PubMed : 22311970, PubMed : 27484799). Has a broad substrate specificity : able to acetylate the initiator methionine of most peptides, except for those with a proline in second position (PubMed : 27484799). Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins (PubMed : 19744929). Autoacetylates in vivo (PubMed : 19744929). The relevance of N-epsilon-acetyltransferase activity is however unclear : able to acetylate H4 in vitro, but this result has not been confirmed in vivo (PubMed : 19744929). Component of N-alpha-acetyltransferase complexes containing NAA10 and NAA15, which has N-alpha-acetyltransferase activity (PubMed : 16507339, PubMed : 27484799, PubMed : 29754825, PubMed : 32042062). Does not influence the acetyltransferase activity of NAA10 (PubMed : 16507339, PubMed : 27484799). However, it negatively regulates the N-alpha-acetyltransferase activity of the N-terminal acetyltransferase A complex (also called the NatA complex) (PubMed : 32042062). The multiprotein complexes probably constitute the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides (PubMed : 16507339, PubMed : 27484799). Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin : may act by counteracting the function of NAA10 (PubMed : 17502424, PubMed : 27422821).

See full target information NAA50

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Neurotherapeutics : the journal of the American So : PubMed31898284

2020

CCR1 Activation Promotes Neuroinflammation Through CCR1/TPR1/ERK1/2 Signaling Pathway After Intracerebral Hemorrhage in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yan,Gang Zuo,Prativa Sherchan,Lei Huang,Umut Ocak,Weilin Xu,Zachary D Travis,Wenna Wang,John H Zhang,Jiping Tang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NAT13 antibody

Immunoprecipitation - Anti-NAT13 antibody (AB241487)

Western blot - Anti-NAT13 antibody (AB241487)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise