Anti-Nav1.8/SCN10A antibody [EPR30868-73]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human iPSC derived ioSensory Neurons (ab317489) cells labelling Nav1.8/SCN10A with ab326327 at 1/200 (2.405 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in human iPSC derived ioSensory neurons (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab78078 Anti-beta III Tubulin mouse monoclonal antibody - Neuronal Marker was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution(Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunohistochemical analysis of paraffin-embedded Human dorsal root ganglion (DRG) tissue labeling Nav1.8/SCN10A with ab326327 at 1/100 (4.81 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human DRG. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunohistochemical analysis of paraffin-embedded Mouse dorsal root ganglion (DRG) tissue labeling Nav1.8/SCN10A with ab326327 at 1/500 (0.962 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse DRG. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunohistochemical analysis of paraffin-embedded
(A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse SCN10A expression vector containing a his tag
(B) HEK-293T transfected with a human SCN10A expression vector containing a his tag
(C) HEK-293T transfected with a mouse SCN3A expression vector containing a his tag
(D) HEK-293T transfected with a mouse SCN4A expression vector containing a his tag
(E) HEK-293T transfected with a mouse SCN5A expression vector containing a his tag
(F) HEK-293T transfected with a mouse SCN9A expression vector containing a his tag
(G) HEK-293T transfected with a mouse SCN11A expression vector containing a his tag
(H) HEK-293T transfected with empty vector containing a his tag
tissues labeling Nav1.8/SCN10A with ab326327 at 1/500 (0.962 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a mouse SCN10A expression vector containing a his tag, (B) HEK-293T transfected with a human SCN10A expression vector containing a his tag, no staining on (C) HEK-293T transfected with a mouse SCN3A expression vector containing a his tag, (D) HEK-293T transfected with a mouse SCN4A expression vector containing a his tag, (E) HEK-293T transfected with a mouse SCN5A expression vector containing a his tag, (F) HEK-293T transfected with a mouse SCN9A expression vector containing a his tag, (G) HEK-293T transfected with a mouse SCN11A expression vector containing a his tag, (H) HEK-293T transfected with empty vector containing a his tag. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Nav1.8/SCN10A with ab326327 at 1/100 (4.81 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human cardiac muscle (PMID : 16750188). The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Nav1.8/SCN10A with ab326327 at 1/500 (0.962 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse spleen. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Nav1.8/SCN10A with ab326327 at 1/500 (0.962 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse liver. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC cells labelling Nav1.8/SCN10A with ab326327 at 1/200 (2.405 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing no staining in human PBMC (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-human CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain CD3 at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.8/SCN10A antibody [EPR30868-73] (AB326327)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Nav1.8/SCN10A with ab326327 at 1/100 (4.81 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human liver (PMID : 16750188). The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins