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AB242220

Anti-NBAS antibody

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Rabbit Polyclonal NBAS antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NBAS aa 350-400.

대체 명칭 보기

NAG, NBAS, NBAS subunit of NRZ tethering complex, Neuroblastoma-amplified gene protein, Neuroblastoma-amplified sequence

2 이미지
Immunoprecipitation - Anti-NBAS antibody (AB242220)
  • IP

Supplier Data

Immunoprecipitation - Anti-NBAS antibody (AB242220)

NBAS was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242220 at 3 μl/reaction.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-NBAS antibody (ab242220) at 1/1000 dilution

Lane 1:

ab242220 IP in Jurkat whole cell lysate

Lane 2:

Control IgG IP in Jurkat whole cell lysate

Predicted band size: 269 kDa

true

Exposure time: 3min

Western blot - Anti-NBAS antibody (AB242220)
  • WB

Supplier Data

Western blot - Anti-NBAS antibody (AB242220)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-NBAS antibody (ab242220) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 269 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NBAS aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

A2RRP1

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대체품

Primary Antibodies

AB122370

Anti-NBAS antibody

Immunocytochemistry/ Immunofluorescence - Anti-NBAS antibody (AB122370)

  • 5
  • 1

보완 제품

Primary Antibodies

AB214671

Anti-NAGLU/NAG antibody [EPR20708]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (AB214671)

  • 0
  • 0
Proteins & Peptides

AB162603

Recombinant Human NBAS protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human NBAS protein (GST tag N-Terminus) (AB162603)

  • 0
  • 0
Primary Antibodies

AB151100

Anti-SPEF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEF1 antibody (AB151100)

  • 5
  • 1
Primary Antibodies

AB200346

Anti-SGSH/HSS antibody [EPR17312]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] (AB200346)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>3 - 6 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Antibody was affinity purified using an epitope specific to NBAS immobilized on solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The NBAS protein also known as Neuroblastoma Amplified Sequence functions as an important player in intracellular trafficking and protein stability. It weighs approximately 237 kDa and is broadly expressed across various tissues with noticeable presence in the liver and brain. The NBAS protein forms part of the syntaxin-binding protein 5 (STXBP5) complex aligning itself with other cytoplasmic and membrane-associated proteins. Through its interaction with these cellular structures the NBAS protein facilitates proper protein transportation within the cells.
Biological function summary

The NBAS protein contributes to maintaining homeostasis by participating in endoplasmic reticulum-to-Golgi apparatus transport. This protein joins the syntaxin 18 complex an assembly of proteins that forms part of the broader secretion machinery in cells. Through this involvement NBAS ensures effective protein sorting and delivery which is critical for cell function and communication. Disruption in NBAS activity might lead to accumulation of unfolded proteins affecting overall cellular health.

Pathways

The NBAS protein plays a significant role in the vesicular transport and secretory pathways. It functions alongside proteins such as STX18 and presumed interactions with other members of the SNARE complex facilitating the fusion of vesicles with target membranes. By participating in these pathways NBAS ensures the smooth transition of proteins and contributes indirectly to processes like secretion and intracellular signaling.

NBAS mutations have been associated with Short Stature Onychodysplasia Facial Dysmorphism and Hypotrichosis (SOFT) syndrome as well as liver dysfunction. SOFT syndrome symptoms indicate the protein's involvement in bone growth and hair follicle development while liver dysfunction suggests its necessity in hepatic protein trafficking. In both cases the NBAS shares connections with proteins in similar pathways such as components of the syntaxin and SNARE family indicating a broader impact on cellular and systemic health when NBAS function is compromised.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Involved in Golgi-to-endoplasmic reticulum (ER) retrograde transport; the function is proposed to depend on its association in the NRZ complex which is believed to play a role in SNARE assembly at the ER (PubMed : 19369418). Required for normal embryonic development (By similarity). May play a role in the nonsense-mediated decay pathway of mRNAs containing premature stop codons (By similarity).
See full target information NBAS
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 6:1211 PubMed38017066

2023

Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline E Strong,Jiajing Zhang,Martin Carrasco,Srikanya Kundu,Molly Boutin,Harshad D Vishwasrao,Jiamin Liu,Angelica Medina,Yu-Chi Chen,Kelli Wilson,Emily M Lee,Marc Ferrer
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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