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AB185234

Anti-NCE2/UBE2F antibody [EPR12932]

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(6 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal NCE2/UBE2F antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 6 publications.
6 이미지
Immunocytochemistry/ Immunofluorescence - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)

Immunofluorescent analysis of A375 cells (4% paraformaldehyde-fixed) labeling NCE2/UBE2F with ab185234 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)

Immunohistochemical analysis of paraffin-embedded Human renal carcinoma labeling NCE2/UBE2F with ab185234 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)

Immunohistochemical analysis of paraffin-embedded Human brain labeling NCE2/UBE2F with ab185234 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)

Intracellular flow cytometric analysis of A375 cells (2% paraformaldehyde-fixed) labeling NCE2/UBE2F with ab185234at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Western blot - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)
  • WB

Lab

Western blot - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)

Lanes 1-3 : Merged signal (red and green). Green - ab185234 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

ab185234 Anti-NCE2/UBE2F antibody [EPR12932] was shown to specifically react with NCE2/UBE2F in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265339 (knockout cell lysate ab257777) was used. Wild-type and NCE2/UBE2F knockout samples were subjected to SDS-PAGE. ab185234 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human UBE2F (NCE2) knockout HeLa cell lysate (<a href='/ko/products/cell-lysates/human-ube2f-nce2-knockout-hela-cell-lysate-ab257777'>ab257777</a>) at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 24 kDa

false

Western blot - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)
  • WB

Supplier Data

Western blot - Anti-NCE2/UBE2F antibody [EPR12932] (AB185234)

All lanes:

Western blot - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

A375 cell lysate at 20 µg

Lane 3:

NTERA2/D1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-NCE2/UBE2F antibody [EPR12932] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12932

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB184177

Anti-Cullin 5/CUL-5 antibody [EPR14725]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Cullin 5/CUL-5 antibody [EPR14725] (AB184177)

  • 0
  • 0
Primary Antibodies

AB81264

Anti-NEDD8 antibody [Y297]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-NEDD8 antibody [Y297] (AB81264)

  • 3
  • 4
Primary Antibodies

AB133565

Anti-RBX1 antibody [EPR6850(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RBX1 antibody [EPR6850(B)] (AB133565)

  • 4
  • 1
Primary Antibodies

AB181986

Anti-RNF7 antibody [EPR12001]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (AB181986)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/160", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NCE2/UBE2F also known simply as UBE2F acts as a ubiquitin-conjugating enzyme that is critical in the process of neddylation. The mass of UBE2F is approximately 21 kDa. It plays a mechanical role in transferring the activated NEDD8 protein to target substrates which include members of the Cullin-RING ligase complexes. Expression of UBE2F is observed in multiple human tissues with notable presence in the lungs and liver. Its activity ensures the proper modification of proteins that depend on neddylation for their function.
Biological function summary

UBE2F functions as an important component in cellular processes by participating in the neddylation pathway. It forms a complex with NEDD8-activating E1 enzymes facilitating the transfer of NEDD8 to specific E3 ligases primarily targeting cullins. Through neddylation UBE2F influences the activation of these ligases impacting protein degradation pathways cell cycle regulation and signal transduction. This functional role positions UBE2F as an important player in maintaining cellular homeostasis.

Pathways

UBE2F integrates into the neddylation and ubiquitination pathways. Neddylation specifically modulates ubiquitin-proteasome system where NEDD8 acts as a regulator of Cullin-RING E3 ligase activity. Through these interactions UBE2F contributes to the regulation of protein turnover alongside ubiquitin itself. Connections in these pathways often involve proteins like CUL5 which works synchronously with UBE2F to ensure proper cell cycle progression and stress response in cells.

UBE2F has associations with cancer development such as lung cancer and neurodegenerative conditions like Parkinson's disease. Its role in cancer ties to abnormal neddylation activity where overactive maintenance of cullin function disrupts cell cycle control. In Parkinson’s UBE2F-related dysregulation affects protein homeostasis and neuronal health. Proteins linked to disorders like UbcH12 for cancer highlight UBE2F's involvement in complex cellular mechanisms that contribute to disease pathophysiology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 19250909, PubMed : 23201271). Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5) (PubMed : 19250909, PubMed : 23201271, PubMed : 23300442). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B (PubMed : 19250909, PubMed : 23201271). Mediates neddylation of the CUL9-RBX1 complex (PubMed : 38605244).. (Microbial infection) Following infection by HIV-1 virus, participates to HIV-1 Vif protein-mediated ubiquitination and degradation of APOBEC3G by mediating neddylation of cullin-5 (CUL5).
See full target information UBE2F

대체 명칭 보기

NCE2, UBE2F, NEDD8-conjugating enzyme UBE2F, NEDD8 carrier protein UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, RING-type E3 NEDD8 transferase UBE2F, Ubiquitin-conjugating enzyme E2 F
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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305907 PubMed38126621

2023

NNMT/1-MNA Promote Cell-Cycle Progression of Breast Cancer by Targeting UBC12/Cullin-1-Mediated Degradation of P27 Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yilei Ma,Xucheng Huang,Yanzhong Wang,Yinjiao Lei,Jinwei Yu,Shaobo Yu,Yuzhen Gao,Jun Yang,Feng Zhao,Haitao Yu,Jin Zeng,Yadong Chu,Min Yang,Guoli Li,Xinyou Xie,Jun Zhang

Nature communications 14:6774 PubMed37891180

2023

N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvia Varland,Rui Duarte Silva,Ine Kjosås,Alexandra Faustino,Annelies Bogaert,Maximilian Billmann,Hadi Boukhatmi,Barbara Kellen,Michael Costanzo,Adrian Drazic,Camilla Osberg,Katherine Chan,Xiang Zhang,Amy Hin Yan Tong,Simonetta Andreazza,Juliette J Lee,Lyudmila Nedyalkova,Matej Ušaj,Alexander J Whitworth,Brenda J Andrews,Jason Moffat,Chad L Myers,Kris Gevaert,Charles Boone,Rui Gonçalo Martinho,Thomas Arnesen

Cell death & disease 11:975 PubMed33184273

2020

Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Lisha Zhou,Jin Zhu,Wangyang Chen,Yanyu Jiang,Tao Hu,Yinxia Wang,Xiaoling Ye,Mengxi Zhan,Chenghao Ji,Zhuoming Xu,Xinran Wang,Yuanlong Gu,Lijun Jia

Cell death & disease 11:400 PubMed32457294

2020

HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Zongpei Guo,Shaozheng Wang,Ying Xie,Yang Han,Sai Hu,Hua Guan,Dafei Xie,Chenjun Bai,Xiaodan Liu,Yongqing Gu,Ping-Kun Zhou,Teng Ma

eLife 8: PubMed31294695

2019

The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaheen Kabir,Justin Cidado,Courtney Andersen,Cortni Dick,Pei-Chun Lin,Therese Mitros,Hong Ma,Seung Hyun Baik,Matthew A Belmonte,Lisa Drew,Jacob E Corn

Molecular cell 70:1008-1024.e6 PubMed29932898

2018

UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F.

Applications

Unspecified application

Species

Unspecified reactive species

Weihua Zhou,Jie Xu,Mingjia Tan,Haomin Li,Hua Li,Wenyi Wei,Yi Sun
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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