AB63554

Anti-NCF1/p47-phox (phospho S304) antibody

Be the first to review this product! 리뷰 제출

(6 제품이 사용된 논문 )

Rabbit Polyclonal NCF1/p47-phox phospho S304 antibody. Suitable for WB and reacts with African green monkey, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human NCF1 pS304.

2 이미지

Unknown

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (AB63554)

All lanes:

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) at 1/500 dilution

Lane 1:

extracts from COS7 cells treatedwith UV (15mins) at 30 µg

Lane 2:

A549 cells extracts at 30 µg

Lane 3:

A549 cells extracts at 30 µg with immunizing peptide

Predicted band size: 44 kDa

Observed band size: 45 kDa

false

CiteAb

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (AB63554)

NCF1/p47-phox (phospho S304) western blot using anti-NCF1/p47-phox (phospho S304) antibody ab63554. Publication image and figure legend from Sowrirajan, B., Saito, Y., et al., 2017, Sci Rep, PubMed 28240310.

ab63554 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab63554 please see the product overview.

The expression of p47phox is increased in IL-27-induced macrophages (I-Mac).Monocytes were differentiated into macrophages in the absence (M-Mac) or presence of IL-27 (I-Mac). After differentiation, cells were incubated with or without IL-27. (a) M-mac, I-Mac, untreated M-Mac and IL-27-treated M-Mac were stimulated with or without 100 ng/ml PMA for 30 min at 37 °C and then ROS activity was measured by detection of secreted H2O2. Data shown represent means ± SD of triplicate samples. (b) The expression levels of p47phox were determined by western blot for M-Mac, I-Mac, untreated M-Mac and IL-27-treated M-Mac. (c) M-Mac and I-Mac were stimulated with or without PMA for 30 min, and then cell lysates were subjected to 2D gel electrophoresis. Expression of p47phox was detected by anti-p47phox and anti-phosphorylated S304 p47phox antibodies. (d) Whole cell lysates of M-Mac and I-Mac were prepared using RIPA buffer. Western blot analyses were performed using anti-SOD1, SOD2, SOD3 or anti-β-actin antibodies. The intensity of the band was analyzed by NIH Image J and normalized each SOD intensity with β-Actin.

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, African green monkey

Applications

applications

Immunogen

Synthetic Peptide within Human NCF1 pS304. The exact immunogen used to generate this antibody is proprietary information.

P14598

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Affinity purified from rabbit antiserum using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NCF1 also known as p47-phox p47-phox protein or NCF-1 is a cytosolic component of the NADPH oxidase complex. It has a molecular mass of about 47 kDa. NCF1/p47-phox is expressed in neutrophils monocytes and other phagocytic cells. Functionally it plays an important role in the regulation of reactive oxygen species (ROS) production by facilitating the translocation of other subunits to the cell membrane where the NADPH oxidase complex assembles and becomes active.

Biological function summary

The NCF1/p47-phox protein plays a critical role in host defense by generating ROS which are essential for microbial killing. NCF1 is an integral part of the NADPH oxidase complex along with other subunits such as p67-phox p40-phox gp91-phox and p22-phox. When stimulated NCF1 interacts with these subunits to form an active enzyme complex that transfers electrons from NADPH to oxygen producing superoxide anion and other ROS.

Pathways

The NCF1/p47-phox component participates mainly in the NADPH oxidase activation pathway and the respiratory burst pathway. NCF1 is connected to the RAC2 GTPase which upon activation stimulates the assembly and activation of the NADPH oxidase complex at the plasma membrane. This pathway is key for the innate immune response and effective clearance of pathogens by phagocytic cells.

Mutations or dysfunction in the NCF1/p47-phox gene can lead to chronic granulomatous disease (CGD) which is characterized by a failure to produce ROS and a consequent inability to kill certain bacteria and fungi. Additionally NCF1 has links to autoimmune conditions such as rheumatoid arthritis where impaired ROS production may contribute to pathogenesis. These disease implications also involve other components of the NADPH oxidase complex such as gp91-phox whose dysfunction similarly contributes to these disorders.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 2547247, PubMed : 2550933, PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed : 12732142, PubMed : 19801500).

See full target information NCF1 pS304

대체 명칭 보기

NOXO2, SH3PXD1A, NCF1, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox

style

left-column

제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Redox biology 74:103231 PubMed38861835

2024

Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Yu Fu,Jiameng Gao,Qingqing Zhang,Nan Zhang,Zhiyuan Zhang,Xuemei Jiang,Chang Chen,Zongmei Wen

Inflammation and regeneration 42:24 PubMed35915511

2022

Diet-induced obesity impairs spermatogenesis: the critical role of NLRP3 in Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Mu,Tai-Lang Yin,Yan Zhang,Jing Yang,Yan-Ting Wu

Frontiers in pharmacology 11:556074 PubMed33658920

2021

D-4F Ameliorates Contrast Media-Induced Oxidative Injuries in Endothelial Cells via the AMPK/PKC Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yansong Guo,Wei Li,Mingming Qian,Ting Jiang,Ping Guo,Qian Du,Na Lin,Xianwei Xie,Zhiyong Wu,Donghai Lin,Donghui Liu

Free radical biology & medicine 152:186-196 PubMed32081748

2020

Geniposide protects against sepsis-induced myocardial dysfunction through AMPKα-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Song,Di-Fei Shen,Yan-Yan Meng,Chun-Yan Kong,Xin Zhang,Yu-Pei Yuan,Ling Yan,Qi-Zhu Tang,Zhen-Guo Ma

Scientific reports 7:43441 PubMed28240310

2017

Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47.

Applications

Unspecified application

Species

Unspecified reactive species

Bharatwaj Sowrirajan,Yoshiro Saito,Deepak Poudyal,Qian Chen,Hongyan Sui,Suk See DeRavin,Hiromi Imamichi,Toyotaka Sato,Douglas B Kuhns,Noriko Noguchi,Harry L Malech,H Clifford Lane,Tomozumi Imamichi

Chemico-biological interactions 189:72-81 PubMed20920494

2010

Lipopolysaccharide-induced cytokine expression in alveolar epithelial cells: role of PKCζ-mediated p47phox phosphorylation.

Applications

Species

Human

Jeremy T Leverence,Meetha Medhora,Girija G Konduri,Venkatesh Sampath

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCF1/p47-phox (phospho S304) antibody

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (AB63554)

Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (AB63554)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise