AB244383

Anti-NCKAP1 antibody

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Rabbit Polyclonal NCKAP1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NCKAP1 aa 900 to C-terminus.

대체 명칭 보기

HEM2, KIAA0587, NAP1, NCKAP1, Nck-associated protein 1, NAP 1, Membrane-associated protein HEM-2, p125Nap1

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-NCKAP1 antibody (AB244383)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NCKAP1 antibody (AB244383)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NCKAP1 (green) using ab244383 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1 antibody (AB244383)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1 antibody (AB244383)

Paraffin-embedded human skeletal muscle tissue stained for NCKAP1 using ab244383 at 1/50 dilution in immunohistochemical analysis.

Low expression as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1 antibody (AB244383)

Paraffin-embedded human cerebral cortex tissue stained for NCKAP1 using ab244383 at 1/50 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NCKAP1 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2A7

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NCKAP1 also known as NAP1 serves an important role mechanically as part of the WAVE regulatory complex. It has a molecular weight of approximately 140 kDa. NCKAP1 is expressed in various tissues with higher levels noted in brain and muscle tissues. It functions in actin cytoskeleton organization by regulating the activation of the Arp2/3 complex which initiates actin polymerization.

Biological function summary

NCKAP1 contributes to cell motility and morphology by participating in lamellipodia and filopodia formation. It forms part of the WAVE complex which includes proteins such as ABI CYFIP and WASP-family proteins. This complex plays a role in rearranging the actin cytoskeleton necessary for various cell movements and shape changes important in processes like cell migration and wound healing.

Pathways

NCKAP1 interacts within the Rac1 signaling pathway. Its actions in the Rac1 pathway are important for membrane ruffling and cell spreading. NCKAP1 is also linked to the mTOR pathway through its role in regulating cytoskeletal dynamics. NCKAP1 activities are associated with other proteins like the p21-activated kinase (PAK) and Filamin A which collaborate in these signaling cascades to execute cellular responses.

NCKAP1 has been implicated in neurological disorders like autism spectrum disorder and schizophrenia. Aberrant actin dynamics regulated by NCKAP1 may contribute to the synaptic dysfunctions observed in these conditions. Furthermore its association with disease-related proteins like CYFIP1 highlights its relevance in these pathologies. Researchers continue to explore how NCKAP1's regulation of the cytoskeleton might influence disease pathways seeking potential therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.

See full target information NCKAP1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PeerJ 12:e16921 PubMed38426148

2024

Identification and immunoinfiltration analysis of key genes in ulcerative colitis using WGCNA.

Applications

Unspecified application

Species

Unspecified reactive species

Siyi Ni,Yingchao Liu,Jihong Zhong,Yan Shen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NCKAP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NCKAP1 antibody (AB244383)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1 antibody (AB244383)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCKAP1 antibody (AB244383)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise