Anti-NCOA3 Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA3 Antibody (AB327204)

Immunohistochemical staining of human placenta using ab327204 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in trophoblastic cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA3 Antibody (AB327204)

Immunohistochemical staining of human skeletal muscle using ab327204 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in myocytes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA3 Antibody (AB327204)

Immunohistochemical staining of human kidney using ab327204 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in cells in glomeruli and cells in tubules.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA3 Antibody (AB327204)

Immunohistochemical staining of human lymph node using ab327204 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in germinal center and non-germinal center cells.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NCOA3 Antibody (AB327204)

Immunofluorescent staining of human cell line A-431 using ab327204 at 0.25–2 µg/ml, showing localization to nucleoplasm & cytosol.

• ChIP

Supplier Data

ChIP - Anti-NCOA3 Antibody (AB327204)

ChIP-Exo-Seq composite graph for ab327204 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-NCOA3 Antibody (AB327204)

Western blot analysis using ab327204 at 0.04–0.4 µg/ml.
Lane 1 : Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2 : Human cell line MCF-7

All lanes:

Western blot - Anti-NCOA3 Antibody (ab327204)

All lanes:

Human cell line MCF-7

Predicted band size: 155.29 kDa

false