Anti-NDE1 antibody [EPR30443-553]

• WB

Supplier Data

Western blot - Anti-NDE1 antibody [EPR30443-553] (AB324144)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen

Lane 3-4 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 2:

HeLa transfected with siRNA specifically targeting NDE1 whole cell lysate at 40 µg

Lane 3:

Human brainstem tissue lysate at 40 µg

Lane 4:

Human spleen tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-NDE1 antibody [EPR30443-553] (AB324144)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HepG2, IMR-90

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 7:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 8:

IMR-90 (human lung fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-NDE1 antibody [EPR30443-553] (AB324144)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-NDE1 antibody [EPR30443-553] (ab324144) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 125s